© 1995 American Heart Association, Inc.
Articles |
Dye Tracers Define Differential Endothelial and Smooth Muscle Coupling Patterns Within the Arteriolar Wall
Presented in part at the annual Experimental Biology meeting, Anaheim, Calif, April 24-28, 1994.
From the Department of Molecular Physiology and Biological Physics, School of Medicine, University of Virginia, Charlottesville.
Correspondence to Dr B.R. Duling, Department of Molecular Physiology and Biological Physics, Box 449, Jordan Hall, University of Virginia, Charlottesville, VA 22908.
Abstract Dye tracers were chosen, based on net charge, chemical structure, and reactive groups, to test for the existence of and to provide novel insight into channel selectivities of junctional pathways connecting smooth muscle and endothelial cells of the arteriolar wall. Dyes were injected into individual smooth muscle or endothelial cells of hamster cheek pouch arterioles using microiontophoresis. Coupling, independent of tracer net charge, was seen both within and between cell layers. Endothelial cells were well coupled by all of the tested dyes. Smooth muscle junctions appeared less effective in dye transfer than endothelial junctions. Lucifer yellow was confirmed to be a poor tracer of smooth muscle gap junctions, and remarkably this dye and other related sulfate-containing molecules interfered with dye movement through smooth muscle but not endothelial junctions. Myoendothelial junctions showed a striking polarity of dye movement, with dye transfer from endothelial to smooth muscle cells but little or no transfer in the reverse direction. Because the dyes have size and charge characteristics similar to those of known cellular second messengers, these findings have important implications for cell-cell signaling in the vessel wall.
Key Words: gap junction • confocal microscopy • fluorescent dyes • microcirculation • intercellular communication
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|
|
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|
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|
|
|
|
 |
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|
|
|
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|
|
|
|
|
|
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|
|
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|
|
|
|
|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
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|
|
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