© 1995 American Heart Association, Inc.
Articles |
Inhibition and Rapid Recovery of Ca2+ Current During Ca2+ Release From Sarcoplasmic Reticulum in Guinea Pig Ventricular Myocytes
From the Laboratory of Physiology, University of Leuven (Belgium).
Correspondence to K.R. Sipido, Laboratory of Physiology, KUL, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium.
Abstract We have investigated the modulation of the L-type
Ca2+ channel by Ca2+ released from the
sarcoplasmic reticulum (SR) in single guinea pig ventricular myocytes
under whole-cell voltage clamp. [Ca2+]i was
monitored by fura 2. By use of impermeant monovalent cations in
intracellular and extracellular solutions, the current through
Na+ channels, K+ channels, nonspecific cation
channels, and the Na+-Ca2+ exchanger was
effectively blocked. By altering the amount of Ca2+ loading
of the SR, the time course of the Ca2+ current
(ICa) could be studied during various amplitudes of
Ca2+ release. In the presence of a large Ca2+
release, fast inhibition of ICa occurred, whereas on
relaxation of [Ca2+]i, fast recovery was
observed. The time course of this transient inhibition of
ICa reflected the time course of
[Ca2+]i. However, the inhibition seen in the
first 50 ms, ie, the time of net Ca2+ release from the SR,
exceeded the inhibition observed later during the pulse, suggesting the
existence of a higher [Ca2+] near the channel during this
time. Transient inhibition of ICa during Ca2+
release was observed to a similar degree at all potentials. It could
still be observed in the presence of intracellular ATP-
-S and of
cAMP. Therefore, we conclude that the modulation of ICa by
Ca2+ release from the SR is not related to
dephosphorylation. It could be related to a reduction in the driving
force and to a direct inhibition of the channel by
[Ca2+]i. The observation that the degree of
inhibition does not depend on membrane potential suggests that the
Ca2+ binding site for this modulation is located outside
the pore. The transient nature of the modulation of ICa by
Ca2+ release will contribute to the recovery of
ICa during prolonged action potentials.
Key Words: Ca2+ channel • heart • Ca2+ release • sarcoplasmic reticulum
This article has been cited by other articles:
 |
|
 |
|
  C. H. Orchard, M. Pasek, and F. Brette The role of mammalian cardiac t-tubules in excitation-contraction coupling: experimental and computational approaches Exp Physiol, May 1, 2009; 94(5): 509 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sims, S. Reisenweber, P. C. Viswanathan, B.-R. Choi, W. H. Walker, and G. Salama Sex, Age, and Regional Differences in L-Type Calcium Current Are Important Determinants of Arrhythmia Phenotype in Rabbit Hearts With Drug-Induced Long QT Type 2 Circ. Res., May 9, 2008; 102(9): e86 - e100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Song, J. C. Shryock, and L. Belardinelli An increase of late sodium current induces delayed afterdepolarizations and sustained triggered activity in atrial myocytes Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2031 - H2039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bito, F. R. Heinzel, L. Biesmans, G. Antoons, and K. R. Sipido Crosstalk between L-type Ca2+ channels and the sarcoplasmic reticulum: alterations during cardiac remodelling Cardiovasc Res, January 15, 2008; 77(2): 315 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Altamirano and D. M. Bers Effect of intracellular Ca2+ and action potential duration on L-type Ca2+ channel inactivation and recovery from inactivation in rabbit cardiac myocytes Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H563 - H573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. D. J. ter Keurs and P. A. Boyden Calcium and Arrhythmogenesis Physiol Rev, April 1, 2007; 87(2): 457 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Antoons, P. G. A. Volders, T. Stankovicova, V. Bito, M. Stengl, M. A. Vos, and K. R. Sipido Window Ca2+ current and its modulation by Ca2+ release in hypertrophied cardiac myocytes from dogs with chronic atrioventricular block J. Physiol., February 15, 2007; 579(1): 147 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Bers Altered Cardiac Myocyte Ca Regulation In Heart Failure. Physiology, December 1, 2006; 21(6): 380 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Leem, W. T. Kim, J. M. Ha, Y. J. Lee, H. C. Seong, H. Choe, Y. J. Jang, J. B. Youm, and Y. E Earm Simulation of Ca2+-activated Cl- current of cardiomyocytes in rabbit pulmonary vein: implications of subsarcolemmal Ca2+ dynamics Phil Trans R Soc A, May 15, 2006; 364(1842): 1223 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fauconnier, A. Lacampagne, J.-M. Rauzier, P. Fontanaud, J.-M. Frapier, O. M. Sejersted, G. Vassort, and S. Richard Frequency-dependent and proarrhythmogenic effects of FK-506 in rat ventricular cells Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H778 - H786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bassani, J. Altamirano, J. L. Puglisi, and D. M. Bers Action potential duration determines sarcoplasmic reticulum Ca2+ reloading in mammalian ventricular myocytes J. Physiol., September 1, 2004; 559(2): 593 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brette, L. Salle, and C. H. Orchard Differential Modulation of L-type Ca2+ Current by SR Ca2+ Release at the T-Tubules and Surface Membrane of Rat Ventricular Myocytes Circ. Res., July 9, 2004; 95(1): e1 - e7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. KLEBER and Y. RUDY Basic Mechanisms of Cardiac Impulse Propagation and Associated Arrhythmias Physiol Rev, April 1, 2004; 84(2): 431 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Reuter, T. Han, C. Motter, K. D. Philipson, and J. I. Goldhaber Mice overexpressing the cardiac sodium-calcium exchanger: defects in excitation-contraction coupling J. Physiol., February 1, 2004; 554(3): 779 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zahradnikova, Z. Kubalova, J. Pavelkova, S. Gyorke, and I. Zahradnik Activation of calcium release assessed by calcium release-induced inactivation of calcium current in rat cardiac myocytes Am J Physiol Cell Physiol, February 1, 2004; 286(2): C330 - C341. [Abstract] [Full Text]
|
|
|
|
|
|
I. Findlay Physiological modulation of inactivation in L-type Ca2+ channels: one switch J. Physiol., January 15, 2004; 554(2): 275 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brette, J.-Y. Le Guennec, and I. Findlay Low-voltage triggering of Ca2+ release from the sarcoplasmic reticulum in cardiac muscle cells Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1544 - C1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Spencer and J. S. K. Sham Effects of Na+/Ca2+ exchange induced by SR Ca2+ release on action potentials and afterdepolarizations in guinea pig ventricular myocytes Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2552 - H2562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brette, A. Lacampagne, L. Salle, I. Findlay, and J.-Y. Le Guennec Intracellular Cs+ activates the PKA pathway, revealing a fast, reversible, Ca2+-dependent inactivation of L-type Ca2+ current Am J Physiol Cell Physiol, August 1, 2003; 285(2): C310 - C318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-B. Shen and A. J. Pappano On the Role of Phosphatase in Regulation of Cardiac L-Type Calcium Current by Cyclic GMP J. Pharmacol. Exp. Ther., May 1, 2002; 301(2): 501 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Bers Calcium and Cardiac Rhythms: Physiological and Pathophysiological Circ. Res., January 11, 2002; 90(1): 14 - 17. [Full Text] [PDF]
|
|
|
|
|
|
D. A. Eisner, H. S. Choi, M. E. Diaz, S. C. O'Neill, and A. W. Trafford Integrative Analysis of Calcium Cycling in Cardiac Muscle Circ. Res., December 8, 2000; 87(12): 1087 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Bers Calcium Fluxes Involved in Control of Cardiac Myocyte Contraction Circ. Res., August 18, 2000; 87(4): 275 - 281. [Full Text] [PDF]
|
|
|
|
|
|
S. Barrere-Lemaire, C. Piot, F. Leclercq, J. Nargeot, and S. Richard Facilitation of L-type calcium currents by diastolic depolarization in cardiac cells: impairment in heart failure Cardiovasc Res, August 1, 2000; 47(2): 336 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G.A. Volders, M. A. Vos, B. Szabo, K. R. Sipido, S.H.M. de Groot, A. P.M. Gorgels, H. J.J. Wellens, and R. Lazzara Progress in the understanding of cardiac early afterdepolarizations and torsades de pointes: time to revise current concepts Cardiovasc Res, June 1, 2000; 46(3): 376 - 392. [Full Text] [PDF]
|
|
|
|
|
|
G. U. Ahmmed, P. H. Dong, G. Song, N. A. Ball, Y. Xu, R. A. Walsh, and N. Chiamvimonvat Changes in Ca2+ Cycling Proteins Underlie Cardiac Action Potential Prolongation in a Pressure-Overloaded Guinea Pig Model With Cardiac Hypertrophy and Failure Circ. Res., March 17, 2000; 86(5): 558 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-k. Wei, H. M. Colecraft, C. D. DeMaria, B. Z. Peterson, R. Zhang, T. A. Kohout, T. B. Rogers, and D. T. Yue Ca2+ Channel Modulation by Recombinant Auxiliary {beta} Subunits Expressed in Young Adult Heart Cells Circ. Res., February 4, 2000; 86(2): 175 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Puglisi, W. Yuan, J. W. M. Bassani, and D. M. Bers Ca2+ Influx Through Ca2+ Channels in Rabbit Ventricular Myocytes During Action Potential Clamp : Influence of Temperature Circ. Res., September 17, 1999; 85 (6): e7 - e16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M Bers and E. Perez-Reyes Ca channels in cardiac myocytes: structure and function in Ca influx and intracellular Ca release Cardiovasc Res, May 1, 1999; 42(2): 339 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sorota Insights into the structure, distribution and function of the cardiac chloride channels Cardiovasc Res, May 1, 1999; 42(2): 361 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Priori, J. Barhanin, R. N. W. Hauer, W. Haverkamp, H. J. Jongsma, A. G. Kleber, W. J. McKenna, D. M. Roden, Y. Rudy, K. Schwartz, et al. Genetic and Molecular Basis of Cardiac Arrhythmias: Impact on Clinical Management Part III Circulation, February 9, 1999; 99(5): 674 - 681. [Full Text] [PDF]
|
|
|
|
 |
|
 S.G. Priori, J. Barhanin, R.N.W. Hauer, W. Haverkamp, H.J. Jongsma, A.G. Kleber, W.J. McKenna, D.M. Roden, Y. Rudy, K. Schwartz, et al. Genetic and molecular basis of cardiac arrhythmias: Impact on clinical management Eur. Heart J., February 1, 1999; 20(3): 174 - 195. [PDF]
|
|
|
|
|
|
K. W Linz and R. Meyer Control of L-type calcium current during the action potential of guinea-pig ventricular myocytes J. Physiol., December 1, 1998; 513(2): 425 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Meyers, T. S. Puri, A. J. Chien, T. Gao, P.-H. Hsu, M. M. Hosey, and G. I. Fishman Sorcin Associates with the Pore-forming Subunit of Voltage-dependent L-type Ca2+ Channels J. Biol. Chem., July 24, 1998; 273(30): 18930 - 18935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Priebe and D. J. Beuckelmann Simulation Study of Cellular Electric Properties in Heart Failure Circ. Res., June 15, 1998; 82(11): 1206 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.A Eisner, A.W Trafford, M.E Dnaz, C.L Overend, and S.C O'Neill The control of Ca release from the cardiac sarcoplasmic reticulum: regulation versus autoregulation Cardiovasc Res, June 1, 1998; 38(3): 589 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R Sipido, E. Carmeliet, and F van de Werf T-type Ca2+ current as a trigger for Ca2+ release from the sarcoplasmic reticulum in guinea-pig ventricular myocytes J. Physiol., April 15, 1998; 508(2): 439 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. McCall, K. S. Ginsburg, R. A. Bassani, T. R. Shannon, M. Qi, A. M. Samarel, and D. M. Bers Ca flux, contractility, and excitation-contraction coupling in hypertrophic rat ventricular myocytes Am J Physiol Heart Circ Physiol, April 1, 1998; 274(4): H1348 - H1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C L Overend, S C O'Neill, and D A Eisner The effect of tetracaine on stimulated contractions, sarcoplasmic reticulum Ca2+ content and membrane current in isolated rat ventricular myocytes J. Physiol., March 15, 1998; 507(3): 759 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R Sipido, T. Stankovicova, W. Flameng, J. Vanhaecke, and F. Verdonck Frequency dependence of Ca2+ release from the sarcoplasmic reticulum in human ventricular myocytes from end-stage heart failure Cardiovasc Res, February 1, 1998; 37(2): 478 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Sipido, M. Maes, and F. Van de Werf Low Efficiency of Ca2+ Entry Through the Na+-Ca2+ Exchanger as Trigger for Ca2+ Release From the Sarcoplasmic Reticulum : A Comparison Between L-Type Ca2+ Current and Reverse-Mode Na+-Ca2+ Exchange Circ. Res., December 19, 1997; 81(6): 1034 - 1044. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Shaw and Y. Rudy Ionic Mechanisms of Propagation in Cardiac Tissue : Roles of the Sodium and L-type Calcium Currents During Reduced Excitability and Decreased Gap Junction Coupling Circ. Res., November 19, 1997; 81(5): 727 - 741. [Abstract] [Full Text]
|
|
|
|
|
|
A. W. Trafford, M. E. Diaz, N. Negretti, and D. A. Eisner Enhanced Ca2+ Current and Decreased Ca2+ Efflux Restore Sarcoplasmic Reticulum Ca2+ Content After Depletion Circ. Res., October 19, 1997; 81(4): 477 - 484. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Kurokawa, S. Adachi-Akahane, and T. Nagao 1,5-Benzothiazepine Binding Domain Is Located on the Extracellular Side of the Cardiac L-Type Ca2+ Channel Mol. Pharmacol., February 1, 1997; 51(2): 262 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.J. Grantham and M.B. Cannell Ca2+ Influx During the Cardiac Action Potential in Guinea Pig Ventricular Myocytes Circ. Res., August 1, 1996; 79(2): 194 - 200. [Abstract] [Full Text]
|
|