Circulation Research, Vol 75, 418-425, Copyright © 1994 by American Heart Association

ARTICLES

Acute changes in myosin heavy chain synthesis rate in pressure versus volume overload

T Imamura, PJ McDermott, RL Kent, M Nagatsu, G Cooper 4th and BA Carabello
Department of Medicine, Medical University of South Carolina, Charleston 29425.

The left ventricular hypertrophy that develops with the volume overload of mitral regurgitation is relatively less than that which develops with the pressure overload of aortic stenosis even when both lesions are severe. The hypertrophy that develops must be the sum of changes in the rate of myocardial protein synthesis and degradation. In the present canine study, we explored early changes in the synthesis rate of myosin heavy chain in response to severe acute pressure overload versus that of the severe acute volume overload of mitral regurgitation. We tested the hypothesis that in acute overload, the rate of protein synthesis would increase less in the volume-overload model than in the pressure-overload model, a potential partial mechanism for the discrepancy in the eventual total amount of hypertrophy that develops in these two lesions. Acute pressure overload was produced by inflating a balloon in the descending aorta, and acute volume overload was produced by using our closed-chest mitral chordal rupture technique. In both models, the hemodynamic lesion that was created was severe. In eight dogs with pressure overload, the average gradient across the balloon was 119.8 +/- 6.1 mm Hg. In six dogs with volume overload, the average regurgitant fraction was 0.67 +/- 0.06. Six other dogs served as controls. The average rate of myosin heavy chain synthesis in control dogs was 2.7 +/- 0.2% per day, virtually identical to the rate we found in the severe volume-overload model. In contrast, the rate was increased in the pressure-overload model by 30% to 3.5 +/- 0.3% per day (P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				B. A. Carabello The Current Therapy for Mitral Regurgitation J. Am. Coll. Cardiol., July 29, 2008; 52(5): 319 - 326. [Abstract] [Full Text] [PDF]

				J. A. Hill and E. N. Olson Cardiac Plasticity N. Engl. J. Med., March 27, 2008; 358(13): 1370 - 1380. [Full Text] [PDF]

				A. Diwan and G. W. Dorn II Decompensation of Cardiac Hypertrophy: Cellular Mechanisms and Novel Therapeutic Targets Physiology, February 1, 2007; 22(1): 56 - 64. [Abstract] [Full Text] [PDF]

				D. Scholz, P. McDermott, M. Garnovskaya, T. N. Gallien, S. Huettelmaier, C. DeRienzo, and G. Cooper IV Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes Cardiovasc Res, August 1, 2006; 71(3): 506 - 516. [Abstract] [Full Text] [PDF]

				N. S. Dhalla, M. R. Dent, P. S. Tappia, R. Sethi, J. Barta, and R. K. Goyal Subcellular Remodeling as a Viable Target for the Treatment of Congestive Heart Failure Journal of Cardiovascular Pharmacology and Therapeutics, March 1, 2006; 11(1): 31 - 45. [Abstract] [PDF]

				M. V. Pancheva, V. S. Panchev, A. V. Suvandjieva, and B. D. Levine Lower body negative pressure vs. lower body positive pressure to prevent cardiac atrophy after bed rest and spaceflight. What caused the controversy? J Appl Physiol, March 1, 2006; 100(3): 1090 - 1090. [Abstract] [Full Text] [PDF]

				B. H. Lorell and B. A. Carabello Left Ventricular Hypertrophy : Pathogenesis, Detection, and Prognosis Circulation, July 25, 2000; 102(4): 470 - 479. [Full Text] [PDF]

				Y. Nagatomo, B. A. Carabello, M. L. Coker, P. J. McDermott, S. Nemoto, M. Hamawaki, and F. G. Spinale Differential effects of pressure or volume overload on myocardial MMP levels and inhibitory control Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H151 - H161. [Abstract] [Full Text] [PDF]

				Y. Nagatomo, B. A. Carabello, M. Hamawaki, S. Nemoto, T. Matsuo, and P. J. McDermott Translational mechanisms accelerate the rate of protein synthesis during canine pressure-overload hypertrophy Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2176 - H2184. [Abstract] [Full Text] [PDF]

				D. G. Simpson, M. Majeski, T. K. Borg, and L. Terracio Regulation of Cardiac Myocyte Protein Turnover and Myofibrillar Structure In Vitro by Specific Directions of Stretch Circ. Res., November 12, 1999; 85 (10): e59 - e69. [Abstract] [Full Text] [PDF]

				W. J. Tuxworth Jr., H. Wada, Y. Ishibashi, and P. J. McDermott Role of load in regulating eIF-4F complex formation in adult feline cardiocytes Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1273 - H1282. [Abstract] [Full Text] [PDF]

				G. Nikcevic, M. C. Heidkamp, M. Perhonen, and B. Russell Mechanical activity in heart regulates translation of alpha -myosin heavy chain mRNA but not its localization Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H2013 - H2019. [Abstract] [Full Text] [PDF]

				T. Matsuo, B. A. Carabello, Y. Nagatomo, M. Koide, M. Hamawaki, M. R. Zile, and P. J. McDermott Mechanisms of cardiac hypertrophy in canine volume overload Am J Physiol Heart Circ Physiol, July 1, 1998; 275(1): H65 - H74. [Abstract] [Full Text] [PDF]

				A. A. Makhlouf and P. J. McDermott Increased expression of eukaryotic initiation factor 4E during growth of neonatal rat cardiocytes in vitro Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H2133 - H2142. [Abstract] [Full Text] [PDF]

				B. D. Levine, J. H. Zuckerman, and J. A. Pawelczyk Cardiac Atrophy After Bed-Rest Deconditioning : A Nonneural Mechanism for Orthostatic Intolerance Circulation, July 15, 1997; 96(2): 517 - 525. [Abstract] [Full Text]

				M. E. Ritchie and M. E. Ritchie Characterization of Human B Creatine Kinase Gene Regulation in the Heart in Vitro and in Vivo J. Biol. Chem., October 11, 1996; 271(41): 25485 - 25491. [Abstract] [Full Text] [PDF]

				R. L. Kent and P. J. McDermott Passive Load and Angiotensin II Evoke Differential Responses of Gene Expression and Protein Synthesis in Cardiac Myocytes Circ. Res., May 1, 1996; 78(5): 829 - 838. [Abstract] [Full Text]

				H. Wada, C. T. Ivester, B. A. Carabello, G. Cooper IV, and P. J. McDermott Translational Initiation Factor eIF-4E J. Biol. Chem., April 5, 1996; 271(14): 8359 - 8364. [Abstract] [Full Text] [PDF]

				C. T. Ivester, W. J. Tuxworth, G. I. Cooper IV, and P. J. McDermott Contraction Accelerates Myosin Heavy Chain Synthesis Rates in Adult Cardiocytes by an Increase in the Rate of Translational Initiation J. Biol. Chem., September 15, 1995; 270(37): 21950 - 21957. [Abstract] [Full Text] [PDF]