Circulation Research, Vol 72, 290-296, Copyright © 1993 by American Heart Association

ARTICLES

Endothelium-dependent relaxation of hypertensive resistance arteries is not impaired under all conditions

J Li and RD Bukoski
Division of General Internal Medicine, University of Texas Medical Branch, Galveston 77550.

Endothelium-dependent relaxation of mesenteric resistance arteries of spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats was studied. Acetylcholine-induced relaxation of SHR vessels precontracted with 10 microM norepinephrine was endothelium dependent and attenuated compared with WKY vessels. The impaired response of SHR vessels was normalized by inhibition of cyclooxygenase with indomethacin. Blockade of nitric oxide synthetase with NG-nitro L- arginine methyl ester (L-NAME) or inhibition of guanylate cyclase with methylene blue attenuated acetylcholine-induced relaxation of norepinephrine-contracted SHR vessels but had no effect on WKY vessels. When vessels were precontracted with 30 nM arginine vasopressin, acetylcholine induced similar degrees of relaxation in both strains. A similar response was detected when lysine vasopressin was used to induce tone. Indomethacin had no effect on relaxation responses of SHR and WKY vessels precontracted with either form of vasopressin. L-NAME and methylene blue partially inhibited acetylcholine-induced relaxation of vasopressin-contracted vessels from both strains. Acetylcholine added at baseline did not induce contraction of vessels from either strain. It is concluded that endothelium-dependent relaxation of SHR resistance arteries is not impaired under all circumstances. Acetylcholine-induced relaxation may be suppressed in SHR resistance arteries when norepinephrine is used to induce contraction as a result of catecholamine-induced production of an endothelium-derived contracting factor. Vasopressin, on the other hand, does not elicit production of this contracting factor and may enhance the vasorelaxant action of acetylcholine in resistance arteries of both strains via actions on endothelial or vascular smooth muscle cells.

This article has been cited by other articles:

				E. B. Savage, R. S. Farivar, and E. J. Okum Cardiac Surgical Physiology Card. Surg. Adult, January 1, 2008; 3(2008): 51 - 76. [Full Text]

				K.-T. Kang, J. C. Sullivan, J. M. Sasser, J. D. Imig, and J. S. Pollock Novel Nitric Oxide Synthase-Dependent Mechanism of Vasorelaxation in Small Arteries From Hypertensive Rats Hypertension, April 1, 2007; 49(4): 893 - 901. [Abstract] [Full Text] [PDF]

				J. Vinten-Johansen, Z.-Q. Zhao, and R. A. Guyton Cardiac Surgical Physiology Card. Surg. Adult, January 1, 2003; 2(2003): 53 - 84. [Full Text]

				D. C. Hatton, Q. Yue, J. Chapman, H. Xue, J. Dierickx, C. Roullet, S. Coste, J. B. Roullet, and D. A. McCarron Blood pressure and mesenteric resistance arterial function after spaceflight J Appl Physiol, January 1, 2002; 92(1): 13 - 17. [Abstract] [Full Text] [PDF]

				C. D. Fike, S. L. Pfister, M. R. Kaplowitz, and J. A. Madden Cyclooxygenase contracting factors and altered pulmonary vascular responses in chronically hypoxic newborn pigs J Appl Physiol, January 1, 2002; 92(1): 67 - 74. [Abstract] [Full Text] [PDF]

				A. C. R. Borges, T. Feres, L. M. Vianna, and T. B. Paiva Effect of Cholecalciferol Treatment on the Relaxant Responses of Spontaneously Hypertensive Rat Arteries to Acetylcholine Hypertension, October 1, 1999; 34(4): 897 - 901. [Abstract] [Full Text] [PDF]

				R. Thuraisingham and A. Raine Maintenance of normal agonist-induced endothelium-dependent relaxation in uraemic and hypertensive resistance vessels Nephrol. Dial. Transplant., January 1, 1999; 14(1): 70 - 75. [Abstract] [PDF]

				H. Y. Qiu, D. Henrion, J. Benessiano, C. Heymes, B. Tournier, and B. I. Levy Decreased Flow-Induced Dilation and Increased Production of cGMP in Spontaneously Hypertensive Rats Hypertension, December 1, 1998; 32(6): 1098 - 1103. [Abstract] [Full Text] [PDF]

				A. Radaelli, L. Mircoli, I. Mori, G. Mancia, and A. U. Ferrari Nitric Oxide–Dependent Vasodilation in Young Spontaneously Hypertensive Rats Hypertension, October 1, 1998; 32(4): 735 - 739. [Abstract] [Full Text] [PDF]

				J. R. Martens, P. Y. Reaves, D. Lu, M. J. Katovich, K. H. Berecek, S. P. Bishop, M. K. Raizada, and C. H. Gelband Prevention of renovascular and cardiac pathophysiological changes in hypertension by angiotensin II type 1 receptor antisense gene therapy PNAS, March 3, 1998; 95(5): 2664 - 2669. [Abstract] [Full Text] [PDF]

				H. I. Chen and C. T. Hu Endogenous nitric oxide on arterial hemodynamics: a comparison between normotensive and hypertensive rats Am J Physiol Heart Circ Physiol, October 1, 1997; 273(4): H1816 - H1823. [Abstract] [Full Text] [PDF]

				R. M. White, C. O. Rivera, and C. B. Davison Differential Contribution of Endothelial Function to Vascular Reactivity in Conduit and Resistance Arteries From Deoxycorticosterone-Salt Hypertensive Rats Hypertension, June 1, 1996; 27(6): 1245 - 1253. [Abstract] [Full Text]

				H. Makynen, M. Kahonen, X. Wu, P. Arvola, and I. Porsti Endothelial Function in Deoxycorticosterone-NaCl Hypertension : Effect of Calcium Supplementation Circulation, March 1, 1996; 93(5): 1000 - 1008. [Abstract] [Full Text]

				R. A. Cohen and P. M. Vanhoutte Endothelium-Dependent Hyperpolarization : Beyond Nitric Oxide and Cyclic GMP Circulation, December 1, 1995; 92(11): 3337 - 3349. [Full Text]

				W. R. Dunn and S. M. Gardiner Structural and Functional Properties of Isolated, Pressurized, Mesenteric Resistance Arteries From a Vasopressin-Deficient Rat Model of Genetic Hypertension Hypertension, September 1, 1995; 26(3): 390 - 396. [Abstract] [Full Text]

				T. A. Bruning, P. C. Chang, M. G.C. Hendriks, P. Vermeij, M. Pfaffendorf, and P. A. van Zwieten In Vivo Characterization of Muscarinic Receptor Subtypes That Mediate Vasodilatation in Patients With Essential Hypertension Hypertension, July 1, 1995; 26(1): 70 - 77. [Abstract] [Full Text]

				A. F. Dominiczak and D. F. Bohr Nitric Oxide and Its Putative Role in Hypertension Hypertension, June 1, 1995; 25(6): 1202 - 1211. [Full Text]

				K. Hayashi, H. Suzuki, and T. Saruta Nitric Oxide Modulates but Does Not Impair Myogenic Vasoconstriction of the Afferent Arteriole in Spontaneously Hypertensive Rats : Studies in the Isolated Perfused Hydronephrotic Kidney Hypertension, June 1, 1995; 25(6): 1212 - 1219. [Abstract] [Full Text]

				K. Bian and R. D. Bukoski Myofilament Calcium Sensitivity of Normotensive and Hypertensive Resistance Arteries Hypertension, January 1, 1995; 25(1): 110 - 116. [Abstract] [Full Text]

				J. R. Cockcroft, P. J. Chowienczyk, N. Benjamin, and J. M. Ritter Preserved Endothelium-Dependent Vasodilatation in Patients with Essential Hypertension N. Engl. J. Med., April 14, 1994; 330(15): 1036 - 1040. [Abstract] [Full Text]