Circulation Research, Vol 70, 1122-1127, Copyright © 1992 by American Heart Association

ARTICLES

Alpha 1-adrenoreceptor blockade reduces the angiotensin II-induced vascular smooth muscle cell DNA synthesis in the rat thoracic aorta and carotid artery

EM van Kleef, JF Smits, JG De Mey, JP Cleutjens, DM Lombardi, SM Schwartz and MJ Daemen
Department of Pathology, University of Limburg, Maastricht, The Netherlands.

We explored effects of alpha 1-adrenoreceptor blockade with prazosin on the increased vascular smooth muscle cell (SMC) DNA synthesis induced by angiotensin II (Ang II) in rats. Ang II was infused with or without prazosin or its solvent. Observations were compared with those in rats receiving saline or solvent. In group A, Ang II was infused for 2 weeks by subcutaneously implanted osmotic minipumps at a rate of 35 ng/100 g per minute. Group B received Ang II together with the alpha 1- adrenoreceptor antagonist prazosin (0.35 micrograms/100 g per minute). Group C received Ang II and 50% dimethyl sulfoxide (DMSO), the solvent of prazosin; group D received 50% DMSO; and group E received 0.9% NaCl (Ang II vehicle). All animals were infused with 5-bromo-2'-deoxyuridine for 2 weeks via separate minipumps to measure DNA synthesis. Ang II significantly increased the fraction of DNA synthesizing SMCs in the media of the thoracic aorta from 0.4 +/- 0.1% (mean +/- SD) in group E (n = 6) to 10.8 +/- 7.0% in group A (n = 8). Addition of prazosin to Ang II reduced the labeling fraction of SMCs to 3.0 +/- 2.2% (group B, n = 9). The remaining SMC DNA synthesis in the prazosin-treated group was probably due to the effects of the solvent of prazosin, i.e., 50% DMSO, since infusion of 50% DMSO alone increased the labeling fraction to 4.1 +/- 2.0% (group D, n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				C. Cavadas, D. Cefai, J. Rosmaninho-Salgado, M. A. Vieira-Coelho, E. Moura, N. Busso, T. Pedrazzini, D. Grand, S. Rotman, B. Waeber, et al. Deletion of the neuropeptide Y (NPY) Y1 receptor gene reveals a regulatory role of NPY on catecholamine synthesis and secretion PNAS, July 5, 2006; 103(27): 10497 - 10502. [Abstract] [Full Text] [PDF]

				R. Villalobos-Molina and M. Ibarra Increased Expression and Function of Vascular {alpha}1D-Adrenoceptors May Mediate The Prohypertensive Effects Of Angiotensin II Mol. Interv., December 1, 2005; 5(6): 340 - 342. [Abstract] [Full Text] [PDF]

				D. H. Damon Sympathetic innervation promotes vascular smooth muscle differentiation Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2785 - H2791. [Abstract] [Full Text] [PDF]

				C. Erami, H. Zhang, J. G. Ho, D. M. French, and J. E. Faber alpha 1-Adrenoceptor stimulation directly induces growth of vascular wall in vivo Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1577 - H1587. [Abstract] [Full Text] [PDF]

				B. A. Waldrop, D. Mastalerz, M. T. Piascik, and G. R. Post alpha 1B- and alpha 1D-Adrenergic Receptors Exhibit Different Requirements for Agonist and Mitogen-Activated Protein Kinase Activation to Regulate Growth Responses in Rat 1 Fibroblasts J. Pharmacol. Exp. Ther., January 1, 2002; 300(1): 83 - 90. [Abstract] [Full Text] [PDF]

				B. C. Berk Vascular Smooth Muscle Growth: Autocrine Growth Mechanisms Physiol Rev, July 1, 2001; 81(3): 999 - 1030. [Abstract] [Full Text] [PDF]

				A. Parenti, L. Brogelli, S. Donnini, M. Ziche, and F. Ledda ANG II potentiates mitogenic effect of norepinephrine in vascular muscle cells: role of FGF-2 Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H99 - H107. [Abstract] [Full Text] [PDF]

				M. J. Servant, P. Coulombe, B. Turgeon, and S. Meloche Differential Regulation of P27Kip1 Expression by Mitogenic and Hypertrophic Factors: Involvement of Transcriptional and Posttranscriptional Mechanisms J. Cell Biol., February 7, 2000; 148(3): 543 - 556. [Abstract] [Full Text] [PDF]

				K. Harada, I. Komuro, T. Sugaya, K. Murakami, and Y. Yazaki Vascular Injury Causes Neointimal Formation in Angiotensin II Type 1a Receptor Knockout Mice Circ. Res., February 5, 1999; 84(2): 179 - 185. [Abstract] [Full Text] [PDF]

				K. P. Makaritsis, H. Gavras, Y. Du, A. V. Chobanian, and P. Brecher {alpha}1-Adrenergic Plus Angiotensin Receptor Blockade Reduces Atherosclerosis in Apolipoprotein E–Deficient Mice Hypertension, December 1, 1998; 32(6): 1044 - 1048. [Abstract] [Full Text] [PDF]

				A. Alexandrov, S. Keffel, M. Goepel, and M. C. Michel Stimulation of alpha 1A-Adrenoceptors in Rat-1 Cells Inhibits Extracellular Signal-Regulated Kinase by Activating p38 Mitogen-Activated Protein Kinase Mol. Pharmacol., November 1, 1998; 54(5): 755 - 760. [Abstract] [Full Text]

				E. J. Su, D. M. Lombardi, J. Siegal, and S. M. Schwartz Angiotensin II Induces Vascular Smooth Muscle Cell Replication Independent of Blood Pressure Hypertension, June 1, 1998; 31(6): 1331 - 1337. [Abstract] [Full Text] [PDF]

				R. J. Dilley and M. I. Nataatmadja Heparin inhibits mesenteric vascular hypertrophy in angiotensin II-infusion hypertension in rats Cardiovasc Res, April 1, 1998; 38(1): 247 - 255. [Abstract] [Full Text] [PDF]

				E. J. Su, D. M. Lombardi, J. Wiener, M. J. A. P. Daemen, M. A. Reidy, and S. M. Schwartz Mitogenic Effect of Angiotensin II on Rat Carotid Arteries and Type II or III Mesenteric Microvessels but Not Type I Mesenteric Microvessels Is Mediated by Endogenous Basic Fibroblast Growth Factor Circ. Res., February 23, 1998; 82(3): 321 - 327. [Abstract] [Full Text] [PDF]

				M. R. Bennett, V. Lindner, D. DeBlois, M. A. Reidy, and S. M. Schwartz Effect of Phosphorothioated Oligonucleotides on Neointima Formation in the Rat Carotid Artery : Dissecting the Mechanism of Action Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 2326 - 2332. [Abstract] [Full Text]

				S. Heeneman, J. F.M. Smits, P. J.A. Leenders, P. M.H. Schiffers, and M. J.A.P. Daemen Effects of Angiotensin II on Cardiac Function and Peripheral Vascular Structure During Compensated Heart Failure in the Rat Arterioscler Thromb Vasc Biol, October 1, 1997; 17(10): 1985 - 1994. [Abstract] [Full Text]

				Y. Du, J. Qiu, S. H. Nelson, and D. H. Wang Regulation of type 1 ANG II receptor in vascular tissue: role of alpha 1-adrenoreceptor Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1224 - R1229. [Abstract] [Full Text] [PDF]

				D. H. Wang, J. Qiu, Z. Hu, and Y. Du Regulation of Type 1 Angiotensin II Receptor in Adrenal Gland : Role of {alpha}1-Adrenoreceptor Hypertension, September 1, 1997; 30(3): 345 - 350. [Abstract] [Full Text]

				M. Kohno, K. Yokokawa, H. Kano, K. Yasunari, M. Minami, T. Hanehira, and J. Yoshikawa Adrenomedullin Is a Potent Inhibitor of Angiotensin II–Induced Migration of Human Coronary Artery Smooth Muscle Cells Hypertension, June 1, 1997; 29(6): 1309 - 1313. [Abstract] [Full Text]

				M. L. Clements and J. E. Faber Mechanical Load Opposes Angiotensin-Mediated Decrease in Vascular {alpha}1-Adrenoceptors Hypertension, May 1, 1997; 29(5): 1165 - 1172. [Abstract] [Full Text]

				L. Liaw, D. M. Lombardi, M. M. Almeida, S. M. Schwartz, D. deBlois, and C. M. Giachelli Neutralizing Antibodies Directed Against Osteopontin Inhibit Rat Carotid Neointimal Thickening After Endothelial Denudation Arterioscler Thromb Vasc Biol, January 1, 1997; 17(1): 188 - 193. [Abstract] [Full Text]

				D. deBlois, D. M. Lombardi, E. J. Su, A. W. Clowes, S. M. Schwartz, and C. M. Giachelli Angiotensin II Induction of Osteopontin Expression and DNA Replication in Rat Arteries Hypertension, December 1, 1996; 28(6): 1055 - 1063. [Abstract] [Full Text]

				D. deBlois, S.M. Schwartz, E.M. van Kleef, J.E. Su, K.A. Griffin, A.K. Bidani, M.J.A.P. Daemen, and D.M. Lombardi Chronic {alpha}1-Adrenoreceptor Stimulation Increases DNA Synthesis in Rat Arterial Wall: Modulation of Responsiveness After Vascular Injury Arterioscler Thromb Vasc Biol, September 1, 1996; 16(9): 1122 - 1129. [Abstract] [Full Text]

				D. deBlois, M. Viswanathan, J.E. Su, A.W. Clowes, J.M. Saavedra, and S.M. Schwartz Smooth Muscle DNA Replication in Response to Angiotensin II Is Regulated Differently in the Neointima and Media at Different Times After Balloon Injury in the Rat Carotid Artery: Role of AT1 Receptor Expression Arterioscler Thromb Vasc Biol, September 1, 1996; 16(9): 1130 - 1137. [Abstract] [Full Text]

				S.-M. Yu, S.-Y. Tsai, J.-H. Guh, F.-N. Ko, C.-M. Teng, and J. T. Ou Mechanism of Catecholamine-Induced Proliferation of Vascular Smooth Muscle Cells Circulation, August 1, 1996; 94(3): 547 - 554. [Abstract] [Full Text]

				E. M. van Kleef, J. Fingerle, and M. J.A.P. Daemen Angiotensin II-Induced Progression of Neointimal Thickening in the Balloon-Injured Rat Carotid Artery Is AT1 Receptor Mediated Arterioscler Thromb Vasc Biol, July 1, 1996; 16(7): 857 - 863. [Abstract] [Full Text]

				S. M. Schwartz, D. deBlois, and E. R. M. O'Brien The Intima : Soil for Atherosclerosis and Restenosis Circ. Res., September 1, 1995; 77(3): 445 - 465. [Full Text]

				M. J.A.P. Daemen and J. G.R. De Mey Regional Heterogeneity of Arterial Structural Changes Hypertension, April 1, 1995; 25(4): 464 - 473. [Abstract] [Full Text]

				H. Zhang and J. E. Faber Trophic Effect of Norepinephrine on Arterial Intima-Media and Adventitia Is Augmented by Injury and Mediated by Different {alpha}1-Adrenoceptor Subtypes Circ. Res., October 26, 2001; 89(9): 815 - 822. [Abstract] [Full Text] [PDF]