Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Circulation Research. 1991;69:483-490

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eghbali, M.
Right arrow Articles by Bhambi, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eghbali, M.
Right arrow Articles by Bhambi, B.

Circulation Research, Vol 69, 483-490, Copyright © 1991 by American Heart Association

ARTICLES

Differential effects of transforming growth factor-beta 1 and phorbol myristate acetate on cardiac fibroblasts. Regulation of fibrillar collagen mRNAs and expression of early transcription factors

M Eghbali, R Tomek, VP Sukhatme, C Woods and B Bhambi
Cardiovascular Institute, Michael Reese Hospital, Chicago, IL.

Cardiac fibroblasts are responsible for synthesis and deposition of fibrillar collagen types I and III. Transforming growth factor-beta 1 (TGF-beta 1) has been proved to increase collagen biosynthesis in various systems, both in vivo and in vitro. We have investigated the effect of TGF-beta 1 on collagen gene expression in cultured cardiac fibroblasts and have compared this effect with that of a mitogenic agent, phorbol myristate acetate (PMA). The regulation of collagen types I and III gene expression was examined by using cDNA probes to rat alpha 2 (I) and mouse alpha 1 (III) procollagens. Quiescent cultured cardiac fibroblasts from rabbit heart were treated with TGF- beta 1 (10-15 ng/ml) and PMA (200 ng/ml). After 24 hours of treatment with TGF-beta 1, the abundance of mRNA for pro-alpha 2 (I) and pro- alpha 1 (III) collagens was increased by 112% (p less than 0.001) and 97% (p = 0.05), respectively, in treated fibroblasts compared with untreated cells. However, PMA-treated cells showed an opposite response: a 42% (p = 0.01) decrease in mRNA levels for pro-alpha 2 (I) collagen was observed. Immunofluorescent staining of cardiac fibroblasts in culture with anti-type I collagen antibody showed that alterations in mRNA levels led to altered collagen synthesis: cellular collagen was relatively increased in TGF-beta 1-treated cells and significantly diminished in PMA-treated cells. The abundance of mRNA for pro-alpha 1 (III) collagen was not affected by PMA treatment.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
H. Shan, Y. Zhang, Y. Lu, Y. Zhang, Z. Pan, B. Cai, N. Wang, X. Li, T. Feng, Y. Hong, et al.
Downregulation of miR-133 and miR-590 contributes to nicotine-induced atrial remodelling in canines
Cardiovasc Res, August 1, 2009; 83(3): 465 - 472.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
C. Matsumoto, T. Hayashi, K. Kitada, C. Yamashita, M. Miyamura, T. Mori, A. Ukimura, M. Ohkita, D. Jin, S. Takai, et al.
Chymase Plays an Important Role in Left Ventricular Remodeling Induced by Intermittent Hypoxia in Mice
Hypertension, July 1, 2009; 54(1): 164 - 171.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
N. Hermida, B. Lopez, A. Gonzalez, J. Dotor, J. J. Lasarte, P. Sarobe, F. Borras-Cuesta, and J. Diez
A synthetic peptide from transforming growth factor-{beta}1 type III receptor prevents myocardial fibrosis in spontaneously hypertensive rats
Cardiovasc Res, February 15, 2009; 81(3): 601 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. Hayashi, C. Yamashita, C. Matsumoto, C.-J. Kwak, K. Fujii, T. Hirata, M. Miyamura, T. Mori, A. Ukimura, Y. Okada, et al.
Role of gp91phox-containing NADPH oxidase in left ventricular remodeling induced by intermittent hypoxic stress
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2197 - H2203.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Xu, J. Pang, H. Yin, M. Li, W. Hao, C. Chen, and J.-M. Cao
Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat
Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2952 - H2958.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Bujak and N. G. Frangogiannis
The role of TGF-{beta} signaling in myocardial infarction and cardiac remodeling
Cardiovasc Res, May 1, 2007; 74(2): 184 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. A. Baudino, W. Carver, W. Giles, and T. K. Borg
Cardiac fibroblasts: friend or foe?
Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1015 - H1026.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Stempien-Otero, A. Plawman, J. Meznarich, T. Dyamenahalli, G. Otsuka, and D. A. Dichek
Mechanisms of Cardiac Fibrosis Induced by Urokinase Plasminogen Activator
J. Biol. Chem., June 2, 2006; 281(22): 15345 - 15351.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
S. Jesmin, S. Zaedi, S. Maeda, C. N. Mowa, I. Sakuma, and T. Miyauchi
Reversal of Elevated Cardiac Expression of TGF{beta}1 and Endothelin-1 in OLETF Diabetic Rats by Long-Acting Calcium Antagonist.
Experimental Biology and Medicine, June 1, 2006; 231(6): 907 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
J. Shigeyama, Y. Yasumura, A. Sakamoto, Y. Ishida, T. Fukutomi, M. Itoh, K. Miyatake, and M. Kitakaze
Increased gene expression of collagen Types I and III is inhibited by {beta}-receptor blockade in patients with dilated cardiomyopathy
Eur. Heart J., December 2, 2005; 26(24): 2698 - 2705.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
K. Iwata, Y. Sawa, S. Kitagawa-Sakakida, N. Kawaguchi, N. Matsuura, T. Nakamura, and H. Matsuda
Gene transfection of hepatocyte growth factor attenuates the progression of cardiac remodeling in the hypertrophied heart
J. Thorac. Cardiovasc. Surg., September 1, 2005; 130(3): 719 - 725.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Rosenkranz
TGF-{beta}1 and angiotensin networking in cardiac remodeling
Cardiovasc Res, August 15, 2004; 63(3): 423 - 432.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Jesmin, I. Sakuma, Y. Hattori, and A. Kitabatake
Role of Angiotensin II in Altered Expression of Molecules Responsible for Coronary Matrix Remodeling in Insulin-Resistant Diabetic Rats
Arterioscler Thromb Vasc Biol, November 1, 2003; 23(11): 2021 - 2026.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
P. Lijnen, V. Petrov, and R. Fagard
Transforming growth factor-{beta}1-mediated collagen gel contraction by cardiac fibroblasts
Journal of Renin-Angiotensin-Aldosterone System, June 1, 2003; 4(2): 113 - 118.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
T. Horio, T. Tokudome, T. Maki, F. Yoshihara, S.-i. Suga, T. Nishikimi, M. Kojima, Y. Kawano, and K. Kangawa
Gene Expression, Secretion, and Autocrine Action of C-Type Natriuretic Peptide in Cultured Adult Rat Cardiac Fibroblasts
Endocrinology, June 1, 2003; 144(6): 2279 - 2284.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart J SupplHome page
M. Pauschinger, K. Chandrasekharan, J. Li, W. Poller, M. Noutsias, C. Tschope, and H.-P. Schultheiss
Inflammation and extracellular matrix protein metabolism: two sides of myocardial remodelling
Eur. Heart J. Suppl., December 1, 2002; 4(suppl_I): I49 - I53.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Tian, J. Liu, P. B. Bitterman, and R. J. Bache
Mechanisms of cytokine induced NO-mediated cardiac fibroblast apoptosis
Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1958 - H1967.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Rosenkranz, M. Flesch, K. Amann, C. Haeuseler, H. Kilter, U. Seeland, K.-D. Schluter, and M. Bohm
Alterations of beta -adrenergic signaling and cardiac hypertrophy in transgenic mice overexpressing TGF-beta 1
Am J Physiol Heart Circ Physiol, September 1, 2002; 283(3): H1253 - H1262.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Y. Akiyama-Uchida, N. Ashizawa, A. Ohtsuru, S. Seto, T. Tsukazaki, H. Kikuchi, S. Yamashita, and K. Yano
Norepinephrine Enhances Fibrosis Mediated by TGF-{beta} in Cardiac Fibroblasts
Hypertension, August 1, 2002; 40(2): 148 - 154.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. Fraccarollo, P. Galuppo, J. Bauersachs, and G. Ertl
Collagen accumulation after myocardial infarction: effects of ETA receptor blockade and implications for early remodeling: Presented in part at the 72nd Scientific Session of the American Heart Association, Atlanta, GA, USA, November 7-10, 1999, and published in abstract form (Circulation 1999;100(Suppl. 1):562)
Cardiovasc Res, June 1, 2002; 54(3): 559 - 567.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
V. V. Petrov, R. H. Fagard, and P. J. Lijnen
Stimulation of Collagen Production by Transforming Growth Factor-{beta}1 During Differentiation of Cardiac Fibroblasts to Myofibroblasts
Hypertension, February 1, 2002; 39(2): 258 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Leicht, W. Briest, A. Holzl, and H.-G. Zimmer
Serum depletion induces cell loss of rat cardiac fibroblasts and increased expression of extracellular matrix proteins in surviving cells
Cardiovasc Res, December 1, 2001; 52(3): 429 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. D. Zimmerman, D. P. Thomas, S. G. Velleman, X. Li, T. R. Hansen, and R. J. McCormick
Time course of collagen and decorin changes in rat cardiac and skeletal muscle post-MI
Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1816 - H1822.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
N. Abdelaziz, F. Colombo, I. Mercier, and A. Calderone
Nitric Oxide Attenuates the Expression of Transforming Growth Factor-{beta}3 mRNA in Rat Cardiac Fibroblasts via Destabilization
Hypertension, August 1, 2001; 38(2): 261 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Burger, M. Benicke, A. Deten, and H.-G. Zimmer
Catecholamines stimulate interleukin-6 synthesis in rat cardiac fibroblasts
Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H14 - H21.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
N.-E. Rhaleb, H. Peng, P. Harding, M. Tayeh, M. C. LaPointe, and O. A. Carretero
Effect of N-Acetyl-Seryl-Aspartyl-Lysyl-Proline on DNA and Collagen Synthesis in Rat Cardiac Fibroblasts
Hypertension, March 1, 2001; 37(3): 827 - 832.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D. E. Dostal
Regulation of Cardiac Collagen : Angiotensin and Cross-Talk With Local Growth Factors
Hypertension, March 1, 2001; 37(3): 841 - 844.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. A. Siwik, P. J. Pagano, and W. S. Colucci
Oxidative stress regulates collagen synthesis and matrix metalloproteinase activity in cardiac fibroblasts
Am J Physiol Cell Physiol, January 1, 2001; 280(1): C53 - C60.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Leicht, N. Greipel, and H.-G. Zimmer
Comitogenic effect of catecholamines on rat cardiac fibroblasts in culture
Cardiovasc Res, November 1, 2000; 48(2): 274 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. A. Siwik, D. L.-F. Chang, and W. S. Colucci
Interleukin-1{beta} and Tumor Necrosis Factor-{alpha} Decrease Collagen Synthesis and Increase Matrix Metalloproteinase Activity in Cardiac Fibroblasts In Vitro
Circ. Res., June 23, 2000; 86(12): 1259 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. Y. Li, C. F. McTiernan, and A. M. Feldman
Interplay of matrix metalloproteinases, tissue inhibitors of metalloproteinases and their regulators in cardiac matrix remodeling
Cardiovasc Res, May 1, 2000; 46(2): 214 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. MacKenna, S. R. Summerour, and F. J. Villarreal
Role of mechanical factors in modulating cardiac fibroblast function and extracellular matrix synthesis
Cardiovasc Res, May 1, 2000; 46(2): 257 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Paradis, N. Dali-Youcef, F. W. Paradis, G. Thibault, and M. Nemer
Overexpression of angiotensin II type I receptor in cardiomyocytes induces cardiac hypertrophy and remodeling
PNAS, January 18, 2000; 97(2): 931 - 936.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. G. Meszaros, A. M. Gonzalez, Y. Endo-Mochizuki, S. Villegas, F. Villarreal, and L. L. Brunton
Identification of G protein-coupled signaling pathways in cardiac fibroblasts: cross talk between Gq and Gs
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C154 - C162.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y. Moriguchi, H. Matsubara, Y. Mori, S. Murasawa, H. Masaki, K. Maruyama, Y. Tsutsumi, Y. Shibasaki, Y. Tanaka, T. Nakajima, et al.
Angiotensin II–Induced Transactivation of Epidermal Growth Factor Receptor Regulates Fibronectin and Transforming Growth Factor-ß Synthesis via Transcriptional and Posttranscriptional Mechanisms
Circ. Res., May 14, 1999; 84(9): 1073 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J. E. Bishop and G. Lindahl
Regulation of cardiovascular collagen synthesis by mechanical load
Cardiovasc Res, April 1, 1999; 42(1): 27 - 44.
[Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Nicoletti and J.-B. Michel
Cardiac fibrosis and inflammation: interaction with hemodynamic and hormonal factors
Cardiovasc Res, March 1, 1999; 41(3): 532 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. J. Peterson, H. Ju, J. Hao, M. Panagia, D. C. Chapman, and I. M.C. Dixon
Expression of Gi-2{alpha} and Gs{alpha} in myofibroblasts localized to the infarct scar in heart failure due to myocardial infarction
Cardiovasc Res, March 1, 1999; 41(3): 575 - 585.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Koudssi, J. E. Lopez, S. Villegas, and C. S. Long
Cardiac Fibroblasts Arrest at the G1/S Restriction Point in Response to Interleukin (IL)-1beta . EVIDENCE FOR IL-1beta -INDUCED HYPOPHOSPHORYLATION OF THE RETINOBLASTOMA PROTEIN
J. Biol. Chem., October 2, 1998; 273(40): 25796 - 25803.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. Bray, A. Agrotis, and A. Bobik
Transforming Growth Factor-ß and Receptor Tyrosine Kinase–Activating Growth Factors Negatively Regulate Collagen Genes in Smooth Muscle of Hypertensive Rats
Hypertension, April 1, 1998; 31(4): 986 - 994.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
N. Shimizu, M. Yoshiyama, T. Omura, A. Hanatani, S. Kim, K. Takeuchi, H. Iwao, and J. Yoshikawa
Activation of mitogen-activated protein kinases and activator protein-1 in myocardial infarction in rats
Cardiovasc Res, April 1, 1998; 38(1): 116 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Pauschinger, A. Doerner, A. Remppis, R. Tannhauser, U. Kuhl, and H.-P. Schultheiss
Differential myocardial abundance of collagen type I and type III mRNA in dilated cardiomyopathy: effects of myocardial inflammation
Cardiovasc Res, January 1, 1998; 37(1): 123 - 129.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
W. W. Brooks, O. H. L. Bing, K. G. Robinson, M. T. Slawsky, D. M. Chaletsky, and C. H. Conrad
Effect of Angiotensin-Converting Enzyme Inhibition on Myocardial Fibrosis and Function in Hypertrophied and Failing Myocardium From the Spontaneously Hypertensive Rat
Circulation, December 2, 1997; 96(11): 4002 - 4010.
[Abstract] [Full Text]

Home page
 HypertensionHome page
S. Kim, H. Wanibuchi, A. Hamaguchi, K. Miura, S. Yamanaka, and H. Iwao
Angiotensin Blockade Improves Cardiac and Renal Complications of Type II Diabetic Rats
Hypertension, November 1, 1997; 30(5): 1054 - 1061.
[Abstract] [Full Text]

Home page
 Cardiovasc ResHome page
H. Ju, S. Zhao, D. S Jassal, and I. M.C Dixon
Effect of AT1 receptor blockade on cardiac collagen remodeling after myocardial infarction
Cardiovasc Res, August 1, 1997; 35(2): 223 - 232.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
K. Yagi, S. Kim, H. Wanibuchi, T. Yamashita, Y. Yamamura, and H. Iwao
Characteristics of Diabetes, Blood Pressure, and Cardiac and Renal Complications in Otsuka Long-Evans Tokushima Fatty Rats
Hypertension, March 1, 1997; 29(3): 728 - 735.
[Abstract] [Full Text]

Home page
 CirculationHome page
A. Calderone, N. Takahashi, N. J. Izzo Jr, C. M. Thaik, and W. S. Colucci
Pressure- and Volume-Induced Left Ventricular Hypertrophies Are Associated With Distinct Myocyte Phenotypes and Differential Induction of Peptide Growth Factor mRNAs
Circulation, November 1, 1995; 92(9): 2385 - 2390.
[Abstract] [Full Text]

Home page
 HypertensionHome page
S. Kim, K. Ohta, A. Hamaguchi, T. Yukimura, K. Miura, and H. Iwao
Angiotensin II Induces Cardiac Phenotypic Modulation and Remodeling In Vivo in Rats
Hypertension, June 1, 1995; 25(6): 1252 - 1259.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. Chang and H. Goldberg
Requirements for Transforming Growth Factor-beta Regulation of the Pro-alpha2(I) Collagen and Plasminogen Activator Inhibitor-1 Promoters
J. Biol. Chem., March 3, 1995; 270(9): 4473 - 4477.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Wang, J. Hao, S. C. Jones, M.-S. Yee, J. C. Roth, and I. M. C. Dixon
Decreased Smad 7 expression contributes to cardiac fibrosis in the infarcted rat heart
Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1685 - H1696.
[Abstract] [Full Text] [PDF]


Circulation Research Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1991 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.