Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents
Circulation Research. 1991;68:1768-1772

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boulanger, C. M.
Right arrow Articles by Luscher, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boulanger, C. M.
Right arrow Articles by Luscher, T. F.

Circulation Research, Vol 68, 1768-1772, Copyright © 1991 by American Heart Association

ARTICLES

Hirudin and nitrates inhibit the thrombin-induced release of endothelin from the intact porcine aorta

CM Boulanger and TF Luscher
Department of Research, University Hospital, Basel, Switzerland.

In intact porcine aorta, endothelium-derived nitric oxide released on thrombin stimulation inhibits the concomitant production of endothelin. Experiments were designed to examine the effect of hirudin (which inactivates thrombin) and the nitrovasodilators nitroglycerin and 3- morpholinosydnonimine on the spontaneous and thrombin-stimulated release of endothelin in intact blood vessels. Endothelin was detected by radioimmunoassay in the incubating medium of intact porcine aortas with endothelium. The spontaneous release of endothelin was not affected by hirudin (0.1 micrograms/ml) but that induced by thrombin (4 units/ml) was prevented. Nitroglycerin (10(-5) M) and the active metabolite of molsidomine, 3-morpholinosydnonimine (10(-5) M), did not modify the basal production of endothelin from the intima of intact porcine aortas. However, the nitrates fully inhibited the release of the peptide induced by thrombin (4 units/ml). The inhibitory effects of both 3-morpholinosydnonimine and nitroglycerin on the thrombin- stimulated release of endothelin were abolished in the presence of an inhibitor of soluble guanylate cyclase, methylene blue (10(-5) M). Thus, the thrombin-stimulated release of endothelin is inhibited by inactivation of thrombin with hirudin or by agents that mimic the effect of endothelium-derived nitric oxide. In contrast, the spontaneous production of endothelin is not modulated by the drugs.


This article has been cited by other articles:


Home page
 CirculationHome page
T. F. Luscher and M. Barton
Endothelins and Endothelin Receptor Antagonists : Therapeutic Considerations for a Novel Class of Cardiovascular Drugs
Circulation, November 7, 2000; 102(19): 2434 - 2440.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Shichiri, M. Yokokura, F. Marumo, and Y. Hirata
Endothelin-1 Inhibits Apoptosis of Vascular Smooth Muscle Cells Induced by Nitric Oxide and Serum Deprivation via MAP Kinase Pathway
Arterioscler Thromb Vasc Biol, April 1, 2000; 20(4): 989 - 997.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Parent and M. Lavallee
Endothelin-dependent effects limit flow-induced dilation of conductance coronary vessels after blockade of nitric oxide formation in conscious dogs
Cardiovasc Res, January 14, 2000; 45(2): 470 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. Thorin, R. Parent, Z. Ming, and M. Lavallee
Contribution of endogenous endothelin to large epicardial coronary artery tone in dogs and humans
Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H524 - H532.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Z. Ming, R. Parent, E. Thorin, and M. Lavallee
Endothelin-Dependent Tone Limits Acetylcholine-Induced Dilation of Resistance Coronary Vessels After Blockade of NO Formation in Conscious Dogs
Hypertension, November 1, 1998; 32(5): 844 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Kirchengast and K. Munter
Endothelin and restenosis
Cardiovasc Res, September 1, 1998; 39(3): 550 - 555.
[Full Text] [PDF]

Home page
 HypertensionHome page
P. Moreau, H. Takase, C. F. Kung, S. Shaw, and T. F. Luscher
Blood Pressure and Vascular Effects of Endothelin Blockade in Chronic Nitric Oxide–Deficient Hypertension
Hypertension, March 1, 1997; 29(3): 763 - 769.
[Abstract] [Full Text]

Home page
 CirculationHome page
J. Frielingsdorf, C. Seiler, P. Kaufmann, G. Vassalli, T. Suter, and O. M. Hess
Normalization of Abnormal Coronary Vasomotion by Calcium Antagonists in Patients With Hypertension
Circulation, April 1, 1996; 93(7): 1380 - 1387.
[Abstract] [Full Text]

Home page
 HypertensionHome page
B. Jiang, S. Morimoto, K. Fukuo, A. Hirotani, M. Tamatani, T. Nakahashi, A. Nishibe, T. Niinobu, S. Hata, S. Chen, et al.
Parathyroid Hormone–Related Protein Inhibits Endothelin-1 Production
Hypertension, March 1, 1996; 27(3): 360 - 363.
[Abstract] [Full Text]

Home page
 CirculationHome page
A. Lerman, D. R. Holmes Jr, M. R. Bell, K. N. Garratt, R. A. Nishimura, and J. C. Burnett Jr
Endothelin in Coronary Endothelial Dysfunction and Early Atherosclerosis in Humans
Circulation, November 1, 1995; 92(9): 2426 - 2431.
[Abstract] [Full Text]

Home page
 CirculationHome page
V. Richard, M. Hogie, M. Clozel, B.-M. Loffler, and C. Thuillez
In Vivo Evidence of an Endothelin-Induced Vasopressor Tone After Inhibition of Nitric Oxide Synthesis in Rats
Circulation, February 1, 1995; 91(3): 771 - 775.
[Abstract] [Full Text]


Circulation Research Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1991 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.