Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Circulation Research. 1990;67:68-77

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hocking, D. C.
Right arrow Articles by Johnson, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hocking, D. C.
Right arrow Articles by Johnson, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

Circulation Research, Vol 67, 68-77, Copyright © 1990 by American Heart Association

ARTICLES

Mechanisms of pulmonary edema induced by tumor necrosis factor-alpha

DC Hocking, PG Phillips, TJ Ferro and A Johnson
Research Service, Veterans Administration Medical Center, Albany, NY 12208.

We tested the hypothesis that human recombinant tumor necrosis factor- alpha (TNF) promotes pulmonary edema by neutrophil-dependent effects on the pulmonary vasculature. The isolated guinea pig lung was perfused with phosphate-buffered Ringer's solution with or without human neutrophils. The infusion of neutrophils (9 x 10(6) total) into lungs isolated after the in vivo administration of TNF (3.2 x 10(5) units/kg) resulted in weight gain (+1.951 +/- 0.311 g versus -0.053 +/- 0.053 g in control) and an increase in the lung (wet-dry)-to-dry weight ratio (8.3 +/- 0.5 versus 6.0 +/- 0.2 in control), indicating the formation of pulmonary edema. The neutrophil-dependent pulmonary edema induced by TNF was associated with a combination of increased capillary permeability (capillary filtration coefficient [Kf,c], 0.170 +/- 0.048 g/min/cm H2O/g at 30 minutes versus 0.118 +/- 0.008 g/min/cm H2O/g at baseline) and increased pulmonary capillary pressure (Ppc, 12.8 +/- 0.8 cm H2O at 60 minutes versus 6.0 +/- 0.3 cm H2O at baseline). The Ppc increase was mediated by thromboxane A2 (TXA2) because the TXA2 synthetase inhibitor Dazoxiben (0.5 mM) prevented the effect (Ppc, 6.7 +/- 0.6 cm H2O at 60 minutes with Dazoxiben), and thromboxane B2 (TXB2) levels were increased in the pulmonary venous effluent (5,244 +/- 599 pg/ml at 60 minutes versus 60 +/- 13 pg/ml at baseline). Studies using WEB-2086 (37 microM), a platelet activating factor (PAF) receptor antagonist, indicated that PAF mediated the increased vascular permeability (Kf,c, 0.107 +/- 0.014 g/min/cm H2O/g at 30 minutes using WEB-2086) and, in part, the increased Ppc (Ppc, 8.4 +/- 0.7 cm H2O at 60 minutes using WEB-2086). In addition, alterations of endothelial peripheral actin bands were noted after TNF administration. The data indicate that TNF induces neutrophil-dependent pulmonary edema associated with increased Ppc (mediated by TXA2 and PAF), increased Kf,c (mediated by PAF), and changes in endothelial peripheral actin bands.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Sumagin, E. Lomakina, and I. H. Sarelius
Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling
Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H969 - H977.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Mehta and A. B. Malik
Signaling Mechanisms Regulating Endothelial Permeability
Physiol Rev, January 1, 2006; 86(1): 279 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Gon, M. R. Wood, W. B. Kiosses, E. Jo, M. G. Sanna, J. Chun, and H. Rosen
S1P3 receptor-induced reorganization of epithelial tight junctions compromises lung barrier integrity and is potentiated by TNF
PNAS, June 28, 2005; 102(26): 9270 - 9275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Gao and J. U. Raj
Role of veins in regulation of pulmonary circulation
Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L213 - L226.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. S. Maxey, R. I. Enelow, B. Gaston, I. L. Kron, V. E. Laubach, and A. Doctor
Tumor necrosis factor-{alpha} from resident lung cells is a key initiating factor in pulmonary ischemia-reperfusion injury
J. Thorac. Cardiovasc. Surg., February 1, 2004; 127(2): 541 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Neumann, N. Gertzberg, and A. Johnson
TNF-{alpha} induces a decrease in eNOS promoter activity
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L452 - L459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Teng, S. Kurata, I. Katoh, G. S. Georgieva, T. Nosaka, C. Mitaka, and T. Imai
Cytokine mRNA expression in unilateral ischemic-reperfused rat lung with salt solution supplemented with low-endotoxin or standard bovine serum albumin
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L137 - L142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Gertzberg, P. Neumann, V. Rizzo, and A. Johnson
NAD(P)H oxidase mediates the endothelial barrier dysfunction induced by TNF-{alpha}
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L37 - L48.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. Guazzi
Alveolar-Capillary Membrane Dysfunction in Heart Failure: Evidence of a Pathophysiologic Role
Chest, September 1, 2003; 124(3): 1090 - 1102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Petrache, A. Birukova, S. I. Ramirez, J. G. N. Garcia, and A. D. Verin
The Role of the Microtubules in Tumor Necrosis Factor-{alpha}-Induced Endothelial Cell Permeability
Am. J. Respir. Cell Mol. Biol., May 1, 2003; 28(5): 574 - 581.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
I. PETRACHE, K. BIRUKOV, A. L. ZAIMAN, M. T. CROW, H. DENG, R. WADGAONKAR, L. H. ROMER, and J. G. N. GARCIA
Caspase-dependent cleavage of myosin light chain kinase (MLCK) is involved in TNF-{alpha}-mediated bovine pulmonary endothelial cell apoptosis
FASEB J, March 1, 2003; 17(3): 407 - 416.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Siflinger-Birnboim and A. Johnson
Protein kinase C modulates pulmonary endothelial permeability: a paradigm for acute lung injury
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L435 - L451.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
M Guazzi, G Pontone, R Brambilla, P Agostoni, and G Reina
Alveolar-capillary membrane gas conductance: a novel prognostic indicator in chronic heart failure
Eur. Heart J., March 2, 2002; 23(6): 467 - 476.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Petrache, A. D. Verin, M. T. Crow, A. Birukova, F. Liu, and J. G. N. Garcia
Differential effect of MLC kinase in TNF-{alpha}-induced endothelial cell apoptosis and barrier dysfunction
Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1168 - L1178.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Bove, P. Neumann, N. Gertzberg, and A. Johnson
Role of ecNOS-derived NO in mediating TNF-induced endothelial barrier dysfunction
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L914 - L922.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
T. Ohtsuka, M. Hamada, G. Hiasa, O. Sasaki, M. Suzuki, Y. Hara, Y. Shigematsu, and K. Hiwada
Effect of beta-blockers on circulating levels of inflammatory and anti-inflammatory cytokines in patients with dilated cardiomyopathy
J. Am. Coll. Cardiol., February 1, 2001; 37(2): 412 - 417.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Ferro, P. Neumann, N. Gertzberg, R. Clements, and A. Johnson
Protein kinase C-alpha mediates endothelial barrier dysfunction induced by TNF-alpha
Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1107 - L1117.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M.-F. Tsan, X. Cao, J. E. White, J. Sacco, and C. Y. Lee
Pertussis Toxin-Induced Lung Edema . Role of Manganese Superoxide Dismutase and Protein Kinase C
Am. J. Respir. Cell Mol. Biol., March 1, 1999; 20(3): 465 - 473.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
N. Janabi, I. Hau, and M. Tardieu
Negative Feedback Between Prostaglandin and {alpha}- and {beta}-Chemokine Synthesis in Human Microglial Cells and Astrocytes
J. Immunol., February 1, 1999; 162(3): 1701 - 1706.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. L. Khimenko, G. J. Bagby, J. Fuseler, and A. E. Taylor
Tumor necrosis factor-alpha in ischemia and reperfusion injury in rat lungs
J Appl Physiol, December 1, 1998; 85(6): 2005 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. Matsumori, K. Ono, R. Nishio, Y. Nose, and S. Sasayama
Amiodarone Inhibits Production of Tumor Necrosis Factor-{alpha} by Human Mononuclear Cells : A Possible Mechanism for its Effect in Heart Failure
Circulation, September 2, 1997; 96(5): 1386 - 1389.
[Abstract] [Full Text]

Home page
 CirculationHome page
A. Matsumori, K. Ono, R. Nishio, H. Igata, B Pharm, T. Shioi, S. Matsui, Y. Furukawa, A. Iwasaki, Y. Nose, et al.
Modulation of Cytokine Production and Protection Against Lethal Endotoxemia by the Cardiac Glycoside Ouabain
Circulation, September 2, 1997; 96(5): 1501 - 1506.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
M. C. Deng, B. Dasch, M. Erren, T. Mollhoff, and H. H. Scheld
Impact of Left Ventricular Dysfunction on Cytokines, Hemodynamics, and Outcome in Bypass Grafting
Ann. Thorac. Surg., July 1, 1996; 62(1): 184 - 190.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
H. I. Pass, D. J. Y. Mew, K. C. Kranda, B. K. Temeck, J. S. Donington, and S. A. Rosenberg
Isolated Lung Perfusion With Tumor Necrosis Factor for Pulmonary Metastases
Ann. Thorac. Surg., June 1, 1996; 61(6): 1609 - 1617.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
R. Rabinovici, G. Feuerstein, F. Abdullah, M. Whiteford, P. Borboroglu, E. Sheikh, D.-R. Phillip, P. Ovadia, L. Bobroski, O. Bagasra, et al.
Locally Produced Tumor Necrosis Factor-{alpha} Mediates Interleukin-2Induced Lung Injury
Circ. Res., February 1, 1996; 78(2): 329 - 336.
[Abstract] [Full Text]

Home page
 Toxicol PatholHome page
H. R. Amouzadeh, C. W. Qualls Jr, J. H. Wyckoff III, G. K. Dzata, S. Sangiah, A. Mauromoustakos, and L. E. Stein
Biochemical and Morphological Alterations in Xylazine-Induced Pulmonary Edema*,{dagger}
Toxicol Pathol, November 1, 1993; 21(6): 562 - 571.
[Abstract] [PDF]

Home page
 International Journal of ToxicologyHome page
M. A. Hollinger
Drug-Induced Lung Toxicity
International Journal of Toxicology, January 1, 1993; 12(1): 31 - 47.
[Abstract] [PDF]


Circulation Research Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1990 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.