Circulation Research, Vol 66, 109-114, Copyright © 1990 by American Heart Association

ARTICLES

Correlation of ventricular area, perimeter, and conotruncal diameter with ventricular mass and function in the chick embryo from stages 12 to 24

BB Keller, N Hu and EB Clark
Dorothy S. and Frederick W. Cook Research Laboratory, Department of Pediatrics, University of Rochester School of Medicine, New York 14642.

Ventricular form and function are interrelated during cardiovascular development. The study of muscle mechanics requires the real-time measurement of length, area, or volume. Because volume measures are not currently possible in the embryonic heart, we tested the hypothesis that end-diastolic (ED) and end-systolic (ES) ventricular perimeter, area, and conotruncal diameter correlate with ventricular mass and function in the stage 12 to stage 24 white Leghorn chick embryo. Video images of the contracting heart were recorded at 60 Hz on 1/2" videotape and studied with a custom image-analysis workstation. ED and ES video fields were selected by maximum and minimum ventricular area and were planimetered for epicardial ventricular perimeter, area, and conotruncal diameter. Data are reported as (mean +/- SEM, n greater than or equal to 8) and were tested by analysis of variance and regression analysis. Heart rate calculated from cycle length increased from 78 +/- 6 beats/min at stage 12 to 162 +/- 5 beats/min at stage 24. ED and ES area increased geometrically versus stage (y = 0.53 - 0.08x + 0.004x2, r = 0.96, p less than 0.001; and y = 0.60 - 0.09x + 0.004x2, r = 0.98, p less than 0.001, respectively). ED and ES perimeter and conotruncal diameter increased linearly versus stage (r = 0.95, p less than 0.001; r = 0.96, p less than 0.001; and r = 0.93, p less than 0.001; r = 0.93, p less than 0.001, respectively). Shortening fraction for each measurement increased from stage 12 to 16 or 18 then decreased.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				J. L. Lucitti, K. Tobita, and B. B. Keller Arterial hemodynamics and mechanical properties after circulatory intervention in the chick embryo J. Exp. Biol., May 15, 2005; 208(10): 1877 - 1885. [Abstract] [Full Text] [PDF]

				K Makikallio, P Jouppila, and J Rasanen Human fetal cardiac function during the first trimester of pregnancy Heart, March 1, 2005; 91(3): 334 - 338. [Abstract] [Full Text] [PDF]

				W. Burggren, S. Khorrami, A. Pinder, and T. Sun Body, eye, and chorioallantoic vessel growth are not dependent on cardiac output level in day 3-4 chicken embryos Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1399 - R1406. [Abstract] [Full Text] [PDF]

				S. Srinivasan, H. S. Baldwin, O. Aristizabal, L. Kwee, M. Labow, M. Artman, and D. H. Turnbull Noninvasive, In Utero Imaging of Mouse Embryonic Heart Development With 40-MHz Echocardiography Circulation, September 1, 1998; 98(9): 912 - 918. [Abstract] [Full Text] [PDF]

				B. B. Keller, M. J. MacLennan, J. P. Tinney, and M. Yoshigi In Vivo Assessment of Embryonic Cardiovascular Dimensions and Function in Day-10.5 to -14.5 Mouse Embryos Circ. Res., August 1, 1996; 79(2): 247 - 255. [Abstract] [Full Text]