Circulation Research, Vol 55, 168-175, Copyright © 1984 by American Heart Association
ARTICLES |
In vitro study on contribution of oxidative metabolism of isolated rabbit heart mitochondria to myocardial reperfusion injury
H Otani, H Tanaka, T Inoue, M Umemoto, K Omoto, K Tanaka, T Sato, T Osako, A Masuda and A Nonoyama
The present study was performed to clarify oxygen-induced damage following myocardial reperfusion, using three mitochondrial preparations from isolated rabbit hearts (nonischemic hearts and those subjected to 40 and 90 minutes of normothermic global ischemia). The viability of mitochondria was evaluated by adenosine triphosphate generation. The extent of mitochondrial injury produced by reactive oxygen metabolism was assessed by the intensity of hydroxyl radical signal detected with electron spin resonance spectroscopy and the reduction of coenzyme Q10 level. The greatest oxygen-induced injury was observed in 40-minute ischemic mitochondria exposed to pure oxygen. The use of superoxide dismutase and catalase satisfactorily prevented the oxygen-induced injury. Moreover, the net adenosine triphosphate generation of the 40-minute ischemic mitochondria was comparable to that of the nonischemic mitochondria without the enzymes. These results suggest that reperfusion of the ischemic myocardium with viable mitochondria is deleterious, because mitochondria are susceptible to injury resulting from oxidative metabolism, and that the use of superoxide dismutase, together with catalase, is beneficial for the restoration of cardiac function after ischemia.
This article has been cited by other articles:
 |
|
 |
|
  M. Khan, S. Varadharaj, L. P. Ganesan, J. C. Shobha, M. U. Naidu, N. L. Parinandi, S. Tridandapani, V. K. Kutala, and P. Kuppusamy C-phycocyanin protects against ischemia-reperfusion injury of heart through involvement of p38 MAPK and ERK signaling Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2136 - H2145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tretter and V. Adam-Vizi Generation of Reactive Oxygen Species in the Reaction Catalyzed by {alpha}-Ketoglutarate Dehydrogenase J. Neurosci., September 8, 2004; 24(36): 7771 - 7778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Becker, T. L. vanden Hoek, Z.-H. Shao, C.-Q. Li, and P. T. Schumacker Generation of superoxide in cardiomyocytes during ischemia before reperfusion Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2240 - H2246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Lucas and L. I. Szweda Declines in mitochondrial respiration during cardiac reperfusion: Age-dependent inactivation of alpha -ketoglutarate dehydrogenase PNAS, June 8, 1999; 96(12): 6689 - 6693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nakanishi, M. Inoue, E. Sugawara, and S. Sano ISCHEMIC AND REPERFUSION INJURY OF CYANOTIC MYOCARDIUM IN CHRONIC HYPOXIC RAT MODEL: CHANGES IN CYANOTIC MYOCARDIAL ANTIOXIDANT SYSTEM J. Thorac. Cardiovasc. Surg., December 1, 1997; 114(6): 1088 - 1096. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Corral-Debrinski, G. Stepien, J. M. Shoffner, M. T. Lott, K. Kanter, and D. C. Wallace Hypoxemia Is Associated With Mitochondrial DNA Damage and Gene Induction: Implications for Cardiac Disease JAMA, October 2, 1991; 266(13): 1812 - 1816. [Abstract] [PDF]
|
|
|
|
 |
|
 A. C. Nulton-Persson and L. I. Szweda Modulation of Mitochondrial Function by Hydrogen Peroxide J. Biol. Chem., June 22, 2001; 276(26): 23357 - 23361. [Abstract] [Full Text] [PDF]
|
|