Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Circulation Research. 1975;36:328-351

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goresky, C. A.
Right arrow Articles by Nadeau, B. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goresky, C. A.
Right arrow Articles by Nadeau, B. E.

Circulation Research, Vol 36, 328-351, Copyright © 1975 by American Heart Association

ARTICLES

Red cell carriage of label: its limiting effect on the exchange of materials in the liver

CA Goresky, GG Bach and BE Nadeau

The red cell membrane is a permeability barrier that limits the equilibration of a variety of solutes between red cell and plasma water. We utilized the multiple indicator dilution technique to investigate the effect of this barrier on the exchange in the liver of a group of tracer substances that are not removed in net fashion from the hepatic circulation: thiourea, urea, and chloride. We demonstrated that, after preequilibration of the label with red cells, a red cell carriage effect appeared (the trapping and translocation of label in the red cells), that this effect was most marked when the permeability of the red cell was relatively low for the substance under consideration (thiourea), and that the effect became small when the permeability of the red cells was large for the exchanging substance (urea and chloride). We developed a theoretical description of the retarding effect of the red cell permeability barrier on the extravascular exchange of label and were able to use this description to obtain estimates of the red cell permeability from the in vivo dilution curves. We examined the effect of plasma injection, of changing the input in such a fashion that the label was not preequilibrated with red cells, and found both experimentally and theoretically, that for substances of low permeability the transit time from these experiments, if multiplied by the total water flow or solute flux, gave an overestimate of both the apparent total volume of distribution and the mass of traced material in the system. This last effect is of great importance for the practical design of many biological experiments. Reliable volume and mass estimates can be made only when the labeled material has been preequilibrated with red cells.


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
L. Liu, E. Mak, R. G. Tirona, E. Tan, P. M. Novikoff, P. Wang, A. W. Wolkoff, and K. S. Pang
Vascular Binding, Blood Flow, Transporter, and Enzyme Interactions on the Processing of Digoxin in Rat Liver
J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 433 - 448.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
R. C. Marshall, P. Powers-Risius, B. W. Reutter, J. P. O'Neil, M. La Belle, R. H. Huesman, and H. F. VanBrocklin
Kinetic Analysis of 18F-Fluorodihydrorotenone as a Deposited Myocardial Flow Tracer: Comparison to 201Tl
J. Nucl. Med., November 1, 2004; 45(11): 1950 - 1959.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. S. Edgerton, S. Cardin, M. Emshwiller, D. Neal, V. Chandramouli, W. C. Schumann, B. R. Landau, L. Rossetti, and A. D. Cherrington
Small Increases in Insulin Inhibit Hepatic Glucose Production Solely Caused by an Effect on Glycogen Metabolism
Diabetes, August 1, 2001; 50(8): 1872 - 1882.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. C. Connolly, L. C. Holste, L. N. Aglione, D. W. Neal, D. B. Lacy, M. S. Smith, M. P. Diamond, A. D. Cherrington, and J.-L. Chiasson
Alterations in basal glucose metabolism during late pregnancy in the conscious dog
Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1166 - E1177.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. A. Goresky, G. G. Bach, A. Simard, A. J. Schwab, and A. Bracht
Uptake of lactate by the liver: effect of red blood cell carriage
Am J Physiol Gastrointest Liver Physiol, May 1, 2000; 278(5): G775 - G788.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. K. Sindelar, K. Igawa, C. A. Chu, J. H. Balcom, D. W. Neal, and A. D. Cherrington
Effect of a selective rise in hepatic artery insulin on hepatic glucose production in the conscious dog
Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E806 - E813.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. K. Sindelar, C. A. Chu, D. W. Neal, and A. D. Cherrington
Interaction of equal increments in arterial and portal vein insulin on hepatic glucose production in the dog
Am J Physiol Endocrinol Metab, November 1, 1997; 273(5): E972 - E980.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
P. H. Hinderling
Red Blood Cells: A Neglected Compartment in Pharmacokinetics and Pharmacodynamics
Pharmacol. Rev., September 1, 1997; 49(3): 279 - 295.
[Abstract] [Full Text] [PDF]


Circulation Research Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1975 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.