Calcium-Dependent Action Potentials Produced by Catecholamines in Guinea Pig Atrial Muscle Fibers Depolarized by Potassium

¹ Department of Pharmacology, University of Connecticut Health Center, Hartford, Connecticut 06112

Atrial muscle fibers of the guinea pig were depolarized and rendered inexcitable by elevation of [K⁺]_o to 22 mM. Isoproterenol, epinephrine, and norepinephrine restored propagated action potentials and contractions to atrial cells without changing the resting potential. The peak value of the intracellularly recorded action potentials varied 28 mv for a 10-fold change in [Ca²⁺]_o. Isoproterenol-induced restoration of action potentials was insensitive to large concentrations of tetrodotoxin (3 x 10^-6M) but antagonized by Mn²⁺ (0.2 mM). These data supported the proposal that the catecholamines restored excitability by increasing membrane conductance to Ca²⁺. In addition to the catecholamines, the divalent cations Ba²⁺, Sr²⁺, and Ca²⁺ restored action potentials to atrial muscle fibers depolarized by elevated K⁺. The effectiveness of the divalent ions in allowing action potentials was inversely related to the estimated hydrated ionic radii. Like the action potentials observed in the presence of isoproterenol, those permitted by Ba²⁺, Sr²⁺, and Ca²⁺ were prevented by Mn²⁺ but insensitive to blockade by tetrodotoxin.

Submitted on April 13, 1970
Accepted on July 1, 1970

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