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(Circulation Research. 2009;105:775.)
© 2009 American Heart Association, Inc.

Integrative Physiology

Dual Pathways of Carbon Monoxide–Mediated Vasoregulation

Modulation by Redox Mechanisms

Brian D. Lamon^*, Frank F. Zhang^*, Nitin Puri, Sergey V. Brodsky, Michael S. Goligorsky, Alberto Nasjletti

From the Departments of Pharmacology (B.D.L., F.F.Z., N.P., A.N.) and Medicine (S.V.B., M.S.G.), New York Medical College, Valhalla.

Correspondence to Brian D. Lamon, PhD, Weill Cornell Medical College of Cornell University, 1300 York Ave, New York, NY 10065. E-mail bdlamon{at}aol.com

Rationale: Vascular tissues produce carbon monoxide (CO) via HO-dependent and HO-independent mechanisms; the former in tandem with biliverdin and iron and the latter as a lone product. CO has been shown to function as both a vasoconstrictor and vasodilator; however, factors that dictate the vasoregulatory phenotype of this gas are unknown.

Objective: We investigated whether CO-mediated vasoconstriction is mechanistically linked to enhanced reactive oxygen species production that masks vasodilatory pathways.

Methods and Results: Sprague–Dawley rat interlobar and interlobular arteries were examined in terms of superoxide (O₂^·–) generation and vascular reactivity in the absence and presence of antioxidants. Both authentic CO and the CO-releasing molecule (CORM)-3 constricted renal arteries and increased O₂^·– production in a dose-dependent manner. The antioxidants tempol, ebselen, and deferoxamine inhibited CO-induced O₂^·– production and converted CO from constrictor to dilator. CO-induced O₂^·– generation was found to involve the activity of multiple oxidases including nitric oxide synthase, NADPH oxidase, xanthine oxidase, and complex IV of the mitochondrial electron chain. Furthermore, inhibition of these enzymes converted CO from constrictor to dilator. Similarly, biliverdin and bilirubin inhibited CO-induced O₂^·– production and vasoconstriction, allowing for a vasodilatory response to CO to be expressed. CO-induced vasoconstriction was dependent on a non-thromboxane agonist of the thromboxane receptor, whereas vasodilatory mechanisms of CO relied on the activation of soluble guanylate cyclase and calcium-gated potassium channels.

Conclusions: CO-induced vasoconstriction involves the generation of reactive oxygen species, which, when negated, allows for the expression of vasodilatory pathways which are masked by the primary oxidative stress response to this gas.

Key Words: carbon monoxide • vascular reactivity • reactive oxygen species • oxidative stress • antioxidant