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(Circulation Research. 2009;105:696.)
© 2009 American Heart Association, Inc.

Integrative Physiology

Bone Marrow Progenitor Cells Induce Endothelial Adherens Junction Integrity by Sphingosine-1-Phosphate–Mediated Rac1 and Cdc42 Signaling

Yidan D. Zhao, Hiroshi Ohkawara, Jalees Rehman, Kishore K. Wary, Stephen M. Vogel, Richard D. Minshall, You-Yang Zhao, Asrar B. Malik

From the Department of Pharmacology and Center for Lung and Vascular Biology (Y.D.Z., H.O., K.K.W., S.M.V., R.D.M., Y.-Y.Z., A.B.M.), University of Illinois College of Medicine, and the Section of Cardiology (J.R.), University of Chicago School of Medicine, Chicago, Ill.

Correspondence to Asrar Malik and Yidan Zhao, Department of Pharmacology, University of Illinois College of Medicine, 835 S Wolcott Avenue, MSB-E403, M/C 868, Chicago, IL 60612. E-mail abmalik{at}uic.edu and yidanzhao@gmail.com

Rationale: Little is known about the contribution of bone marrow–derived progenitor cells (BMPCs) in the regulation endothelial barrier function as defined by microvascular permeability alterations at the level of adherens junctions (AJs).

Objective: We investigated the role of BMPCs in annealing AJs and thereby in preventing lung edema formation induced by endotoxin (LPS).

Methods and Results: We observed that BMPCs enhanced basal endothelial barrier function and prevented the increase in pulmonary microvascular permeability and edema formation in mice after LPS challenge. Coculture of BMPCs with endothelial cells induced Rac1 and Cdc42 activation and AJ assembly in endothelial cells. However, transplantation of BMPCs isolated from sphingosine kinase-1–null mice (SPHK1^–/–), having impaired S1P production, failed to activate Rac1 and Cdc42 or protect the endothelial barrier.

Conclusions: These results demonstrate that BMPCs have the ability to reanneal endothelial AJs by paracrine S1P release in the inflammatory milieu and the consequent activation of Rac-1 and Cdc42 in endothelial cells.

Key Words: progenitor cells • endothelial permeability • S1P signaling • RhoGTPases

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Plugging Vascular Leak by Sphingosine Kinase From Bone Marrow Progenitor Cells

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				T. Hla Plugging Vascular Leak by Sphingosine Kinase From Bone Marrow Progenitor Cells Circ. Res., September 25, 2009; 105(7): 614 - 616. [Full Text] [PDF]