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(Circulation Research. 2009;105:965.)
© 2009 American Heart Association, Inc.

Integrative Physiology

Nitro-Fatty Acid Inhibition of Neointima Formation After Endoluminal Vessel Injury

Marsha P. Cole^*, Tanja K. Rudolph^*, Nicholas K.H. Khoo, Uche N. Motanya, Franca Golin-Bisello, Jeffrey W. Wertz, Francisco J. Schopfer, Volker Rudolph, Steven R. Woodcock, Subhashini Bolisetty, Muhammad S. Ali, Jifeng Zhang, Y. Eugene Chen, Anupam Agarwal, Bruce A. Freeman, Philip M. Bauer

From the Departments of Pharmacology & Chemical Biology (M.P.C., T.K.R., N.K.H.K., U.N.M., F.G.-B., F.J.S., V.R., S.R.W., M.S.A., B.A.F., P.M.B.) and Surgery (J.W.W., P.M.B.), University of Pittsburgh, Pa; Cardiovascular Center (J.Z., Y.E.C.), Department of Internal Medicine, University of Michigan, Ann Arbor; and Department of Medicine (S.B., A.A.), Nephrology Research and Training Center, Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham.

Correspondence to Philip M. Bauer, PhD, Department of Surgery, Biomedical Science Tower 3 Room 6058, 3501 5th Ave, Pittsburgh, PA 15261; E-mail bauerpm{at}upmc.edu or to Bruce Freeman, PhD, Department of Pharmacology & Chemical Biology, E1340 Thomas E Starzl Biomedical Science Tower, 200 Lothrop St, Pittsburgh, PA 15261. E-mail freerad@pitt.edu

Rationale: Fatty acid nitroalkenes are endogenously generated electrophilic byproducts of nitric oxide and nitrite-dependent oxidative inflammatory reactions. Existing evidence indicates nitroalkenes support posttranslational protein modifications and transcriptional activation that promote the resolution of inflammation.

Objective: The aim of this study was to assess whether in vivo administration of a synthetic nitroalkene could elicit antiinflammatory actions in vivo using a murine model of vascular injury.

Methods and Results: The in vivo administration (21 days) of nitro-oleic acid (OA-NO₂) inhibited neointimal hyperplasia after wire injury of the femoral artery in a murine model (OA-NO₂ treatment resulted in reduced intimal area and intima to media ratio versus vehicle- or oleic acid (OA)-treated animals,P<0.0001). Increased heme oxygenase (HO)-1 expression accounted for much of the vascular protection induced by OA-NO₂ in both cultured aortic smooth muscle cells and in vivo. Inhibition of HO by Sn(IV)-protoporphyrin or HO-1 small interfering RNA reversed OA-NO₂–induced inhibition of platelet-derived growth factor-stimulated rat aortic smooth muscle cell migration. The upregulation of HO-1 expression also accounted for the antistenotic actions of OA-NO₂ in vivo, because inhibition of neointimal hyperplasia following femoral artery injury was abolished in HO-1^–/– mice (OA-NO₂–treated wild-type versus HO-1^–/– mice, P=0.016).

Conclusions: In summary, electrophilic nitro-fatty acids induce salutary gene expression and cell functional responses that are manifested by a clinically significant outcome, inhibition of neointimal hyperplasia induced by arterial injury.

Key Words: fatty acids • arteries • stenosis • nitric oxide