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(Circulation Research. 2009;105:16.)
© 2009 American Heart Association, Inc.

Molecular Medicine

Peroxisome Proliferator-Activated Receptor Regulates Extracellular Matrix and Apoptosis of Vascular Smooth Muscle Cells Through the Activation of Transforming Growth Factor-β1/Smad3

Hyo Jung Kim, Min Young Kim, Hana Jin, Hyun Joon Kim, Sang Soo Kang, Hye Jung Kim, Jae Heun Lee, Ki Churl Chang, Jin-Yong Hwang, Chihiro Yabe-Nishimura, Jin-Hoi Kim, Han Geuk Seo

From the Department of Pharmacology (Hyo Jung Kim, M.Y.K., H.J., Hye Jung Kim, J.H.L., K.C.C., H.G.S.), Anatomy (H.J.K., S.S.K.), and Internal Medicine (J.-Y.H.), Gyeongsang Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, Korea; Department of Pharmacology (C.Y.-N.), Kyoto Prefectural University of Medicine, Japan; and Department of Animal Biotechnology (J.-H.K.), Kon-Kuk University, Seoul, Korea.

Correspondence to Han Geuk Seo, Department of Pharmacology, Gyeongsang National University School of Medicine, 92 Chilam-Dong, Jinju 660-751, Korea. E-mail hgseo{at}gnu.ac.kr

Homeostasis of the extracellular matrix and apoptosis of vascular smooth muscle cells (VSMCs) are key components in the regulation of the stability of atherosclerotic plaques. Here, we demonstrate that peroxisome proliferator-activated receptor (PPAR) regulates extracellular matrix synthesis and degradation through transforming growth factor-β1 and its effector, Smad3. Activation of PPAR strongly amplified the expression of types I and III collagen, fibronectin, elastin, and TIMP-3 (tissue inhibitor of metalloproteinases 3), but not of TIMP-1, matrix metalloproteinase-2 or -9. The effect of PPAR on the expression of type III collagen was dually regulated by the direct binding of PPAR and Smad3 to a direct repeat-1 site and a Smad-binding element, respectively, in the type III collagen gene promoter. The activation of PPAR attenuated apoptotic cell death in VSMCs induced by oxidized low-density lipoprotein, and similar antiapoptotic effects were observed on treatment of cells with exogenous type I and/or III collagen. Administration of a PPAR ligand GW501516 to mice also suppressed elastase-induced cell death of aortic VSMCs. These results suggest that PPAR-induced upregulation of extracellular matrix proteins exerts an antiapoptotic effect, thereby maintaining the stability of atherosclerotic plaques. Specific ligands of PPAR may aid in the therapeutic intervention of atherosclerosis by improving plaque stability and patient prognosis.

Key Words: collagen • extracellular matrix • peroxisome proliferator-activated receptor • transforming growth factor-β1 • vascular smooth muscle cells