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(Circulation Research. 2008;102:310.)
© 2008 American Heart Association, Inc.

Molecular Medicine

Protein Carbonylation as a Novel Mechanism in Redox Signaling

Chi Ming Wong, Amrita K. Cheema, Lihua Zhang, Yuichiro J. Suzuki

From the Department of Pharmacology (C.M.W., Y.J.S.) and Lombardi Comprehensive Cancer Center (A.K.C., L.Z.), Georgetown University Medical Center, Washington, DC.

Correspondence to Dr Yuichiro J. Suzuki, Department of Pharmacology, Georgetown University Medical Center, NW403 Medical–Dental Building, 3900 Reservoir Rd NW, Washington, DC 20057. E-mail ys82{at}georgetown.edu

Reactive oxygen species serve as second messengers for signal transduction; however, molecular targets of oxidant signaling have not been defined. Here, we show that ligand–receptor–mediated signaling promotes reactive oxygen species–dependent protein carbonylation. Treatment of pulmonary artery smooth muscle cells with endothelin-1 increased protein carbonyls. Carbonylation of the majority of proteins occurred transiently, suggesting that there is also a mechanism for decarbonylation induced by endothelin-1. Decarbonylation was suppressed by inhibition of thioredoxin reductase, and cellular thioredoxin was upregulated during the decarbonylation phase. These results indicate that endothelin-1 promotes oxidant signaling as well as thioredoxin-mediated reductive signaling to regulate carbonylation and decarbonylation mechanisms. In cells treated with endothelin receptor antagonists, hydrogen peroxide scavengers, or an iron chelator, we identified, via mass spectrometry, proteins that are carbonylated in a receptor- and Fenton reaction–dependent manner, including annexin A1, which promotes apoptosis and suppresses cell growth. Carbonylation of annexin A1 by endothelin-1 was followed by proteasome-dependent degradation of this protein. We propose that carbonylation and subsequent degradation of annexin A1 may play a role in endothelin-mediated cell growth and survival, important events in pulmonary vascular remodeling. Protein carbonylation in response to ligand–receptor interactions represents a novel mechanism in redox signaling.

Key Words: endothelin-1 • protein carbonylation • oxidant signaling • pulmonary hypertension • smooth muscle

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