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(Circulation Research. 2008;102:1502.)
© 2008 American Heart Association, Inc.

Molecular Medicine

The LIM Protein Leupaxin Is Enriched in Smooth Muscle and Functions As an Serum Response Factor Cofactor to Induce Smooth Muscle Cell Gene Transcription

Liisa J. Sundberg-Smith, Laura A. DiMichele, Rebecca L. Sayers, Christopher P. Mack, Joan M. Taylor

From the Departments of Pathology (L.J.S.-S., L.A.D., C.P.M., J.M.T.) and Physiology (R.L.S.) and the Carolina Cardiovascular Biology Center (C.P.M., J.M.T.), University of North Carolina, Chapel Hill.

Correspondence to Joan M. Taylor, Department of Pathology and Laboratory Medicine, 501 Brinkhous-Bullitt Bld CB 7525, University of North Carolina, Chapel Hill, NC 27599-7525. E-mail jmt3x{at}med.unc.edu

Leupaxin is a LIM domain–containing adapter protein belonging to the paxillin family that has been previously reported to be preferentially expressed in hematopoietic cells. Herein, we identified leupaxin in a screen for focal adhesion kinase binding partners in aortic smooth muscle, and we show that leupaxin is enriched in human and mouse vascular smooth muscle and that leupaxin expression is dynamically regulated during development. In addition, our studies reveal that leupaxin can undergo cytoplasmic/nuclear shuttling and functions as an serum response factor cofactor in the nucleus. We found that leupaxin forms a complex with serum response factor and associates with CArG-containing regions of smooth muscle promoters and that ectopic expression of leupaxin induces smooth muscle marker gene expression in both 10T1/2 cells and rat aortic smooth muscle cells. Subsequent studies indicated that enhanced focal adhesion kinase activity (induced by fibronectin or expression of constitutively active focal adhesion kinase) attenuates the nuclear accumulation of leupaxin and limits the ability of leupaxin to enhance serum response factor–dependent gene transcription. Thus, these studies indicate that modulation of the subcellular localization of serum response factor cofactors is 1 mechanism by which extracellular matrix–dependent signals may regulate phenotypic switching of smooth muscle cells.

Key Words: smooth muscle • differentiation • LIM proteins • adhesion • signal transduction

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