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(Circulation Research. 2007;100:1379.)
© 2007 American Heart Association, Inc.

Integrative Physiology

Endothelial Progenitor Cells Stimulate Cerebrovascular Production of Prostacyclin By Paracrine Activation of Cyclooxygenase-2

Anantha Vijay R. Santhanam, Leslie A. Smith, Tongrong He, Karl A. Nath, Zvonimir S. Katusic

From the Departments of Anesthesiology and Molecular Pharmacology & Experimental Therapeutics (A.V.R.S., L.A.S., T.H., Z.S.K.); and Department of Internal Medicine (K.A.N.), Division of Nephrology and Hypertension, Mayo Clinic College of Medicine, Rochester, Minn.

Correspondence to Zvonimir S. Katusic, Department of Anesthesiology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905. E-mail katusic.zvonimir{at}mayo.edu

In the present study we hypothesized that endothelial progenitor cells (EPCs) enhance production of vasoprotective substances in cerebral arteries. Isolated mononuclear cells from rabbit peripheral blood were cultured in endothelial growth medium (EGM-2) for 7 days to yield EPCs. Rabbit basilar arteries were exposed to autologous EPCs (5x10⁵ cells) in vitro or in vivo. Twenty-four hours after intracisternal delivery of autologous EPCs, basilar arteries were isolated and expression of vasoregulatory proteins, production of prostacyclin (PGI₂), and cAMP were determined. Arteries transplanted with EPCs demonstrated increased protein expression of cyclooxygenase-2 and PGI₂ in adventitia, media, and endothelium. Furthermore, production of PGI₂ and arterial content of cAMP, second messenger for PGI₂, were significantly augmented after transplantation of EPCs. In contrast, production of thromboxane A₂ was significantly reduced, whereas production of prostaglandin E₂ remained unchanged. The increased production of PGI₂ and arterial content of cAMP were inhibited only by a selective cyclooxygenase-2 inhibitor, NS-398. In vitro or in vivo treatment of basilar artery with conditioned media from EPCs also caused increase in cyclooxygenase-2 and PGI₂ synthase protein expression associated with elevation of cAMP. Our results suggest that in cerebral arteries, paracrine effect of EPCs promotes vasoprotection by increasing PGI₂ production and intracellular concentration of cAMP. This effect appears to be mediated by activation of arachidonic acid metabolism via stimulation of cyclooxygenase-2/PGI₂ synthase pathway.

Key Words: PGI₂ synthase • vasodilatation • cerebral vasospasm • COX-2 inhibitors • cell therapy

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